07-1673

Anti-SOX1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): SRY (sex determining region Y)-box 1, SRY-related HMG-box gene 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC
• Citations: 2

Eigenschaften

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): mouse
• technique(s): immunohistochemistry: suitable (paraffin)
• NCBI accession no.: NM_009233
• UniProt accession no.: O00570
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SOX1(6656), SRY(6736)

Beschreibung

General description

Sox-1 protein (SOX1), also known as SRY (Sex determining region Y)-box 1, is expressed in the developing brain. SOX1 is related to the HMG box of family transcription factors, which are a highly motile group, with a highly conserved DNA binding domain. Sox1 utilizes multiple pathways to play a direct role on cell fate. SOX1 is also directly involved with sex determination of the Y chromosome. Binding of the SOX1, C-terminus, to the Hes 1 promoter results in Hes1 suppression and attenuation of Notch signaling. When the C-terminus of SOX1 binds to β-catenin, TCF/LEF is suppressed, resulting in the attenuation of Wnt signaling.

Immunogen

Synthetic peptide corresponding to SOX1.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-SOX1 Antibody (Rabbit Polyclonal Antibody) validated in IHC(P) to detect SOX1 also known as SRY (sex determining region Y)-box 1, SRY-related HMG-box gene 1.

Biochem/physiol Actions

This antibody recognizes SOX1.

Physical form

Affinity purified

Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Preparation Note

Stable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10 μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

Analysis Note

Evaluated by immunohistochemistry on human epidermis.

Immunohistochemistry(paraffin):
A 1:300 dilution of this antibody was used to detect SOX1 in human epidermis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable