07-1318

Anti-phospho-JAK2 (Tyr317) Antibody

serum, from rabbit

• Synonym(e): Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), tyrosine-protein kinase JAK2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human
• Application: WB
• Citations: 1

Eigenschaften

• biological source: rabbit
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): western blot: suitable
• NCBI accession no.: NP_004963.1
• UniProt accession no.: O60674
• shipped in: wet ice
• target post-translational modification: phosphorylation (pTyr317)
• Gene Information: human ... JAK2(3717)

Beschreibung

General description

130 kDa

JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.

Immunogen

Epitope: Tyr317

KLH-conjugated recombinant protein selected from residues corresponding to Tyr317 of JAK2.

Application

Detect phospho-JAK2 (Tyr317) using this Anti-phospho-JAK2 (Tyr317) Antibody validated for use in WB.

Research Category
Signaling

Biochem/physiol Actions

This antibody recognizes phosphorylated JAK2.

Physical form

Rabbit serum with 0.05% sodium azide.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blot in JAK2 IP lysate (see below).
Western Blot Analysis: A 1:500 dilution of this antibody detected phospho-JAK2 (Tyr317) on JAK2 IP lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1