04-856

Anti-TBK1 Antibody, clone AOW9, rabbit monoclonal

culture supernatant, clone AOW9, from rabbit

• Synonym(e): NF-kB-activating kinase, NF-kappa-B-activating kinase, TANK-binding kinase 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: AOW9, monoclonal
• Application: IP, WB
• Citations: 9

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: AOW9, monoclonal
• species reactivity: mouse, canine, human, chicken
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_037386
• UniProt accession no.: Q9UHD2
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... TBK1(29110) ; mouse ... Tbkbp1(73174)

Beschreibung

General description

85 kDa

TBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.

Immunogen

Epitope: a.a. 642-645

Peptide corresponding to the sequence C...EEVS... (amino acids 642-645) of human TANK-binding kinase 1 (TBK1) with an N-terminal cysteine added for conjugation purposes.

Application

Detect TBK1 using this Anti-TBK1 Antibody, clone AOW9 validated for use in WB & IP.

Immunoprecipitation:
1-4 μL of a previous lot immunoprecipitated TBK1 from 100 μg of HEK293 RIPA lysate.

Research Category
Signaling

Apoptosis & Cancer

Research Sub Category
Kinases & Phosphatases

Immunological Signaling

Biochem/physiol Actions

Predicted to cross-react with mouse, dog, and chicken based on sequence homology.

TBK1; Does not cross-react with IKKε, IKKα or IKKβ.

Physical form

Cultured supernatant containing 0.05% sodium azide.

Preparation Note

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
RIPA lysates from HEK293 cells.

Evaluated by western blot on RIPA lysates from HEK293 cells.

Western Blot Analysis:
A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.

Other Notes

Replaces: 05-856

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1