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77332

Sigma-Aldrich

Peroxidase from horseradish

lyophilized, powder, ~150 U/mg

Sinónimos:

(2R,3S,6S,7R,8R)-3-(3-formamido-2-hydroxybenzamido)-8-hexyl-2,6-dimethyl-4,9-dioxo-1,5-dioxonan-7-yl 3-methylbutanoate, Antimycin A, Horseradish peroxidase

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100 MG
$140.000
500 MG
$508.000

$140.000

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100 MG
$140.000
500 MG
$508.000

About This Item

Número de CAS:
9003-99-0
Comisión internacional de enzimas:
1.11.1.7 (BRENDA, IUBMB)
Número CE:
232-668-6
Número MDL:
MFCD00071339
Código UNSPSC:
12352204
NACRES:
NA.54

$140.000

En stockDetalles

origen biológico

horseradish

Nivel de calidad

100

Formulario

powder

calidad

lyophilized

actividad específica

~150 U/mg

mol peso

Mr ~40000

solubilidad

50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA)

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

cadena SMILES

[O+H2]O[O-]

InChI

1S/H2O3/c1-3-2/h1-2H

Clave InChI

JSPLKZUTYZBBKA-UHFFFAOYSA-N

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Descripción general

Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays.[1]

Aplicación

Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry.
Peroxidase from horseradish has been used:
  • for isolation of N-Linked glycans[2]
  • as a constituent of D-amino acid oxidase (DAAO) buffer[3]
  • to decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS)[4]

Definición de unidad

1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C

Otras notas

Use of chemically modified peroxidase in benzene[5]; Cross-linking of proteins[6]; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater[7]

sustituido por

Referencia del producto
Descripción
Precios

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P284 - P501

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
BCCD8779
BCBV2718
BCBV3747
BCBP5939V

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Visite la Librería de documentos

Portable oxidative stress sensor: dynamic and non-invasive measurements of extracellular H2O2 released by algae
Koman VB, et al.
Biosensors And Bioelectronics, 68, 245-252 (2015)
Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans
Le HT, et al.
Journal of Analytical Methods in Chemistry, 2018 (2018)
K. Takahashi et al.
Biochemical and Biophysical Research Communications, 121, 216-216 (1984)
J.A. Nicell et al.
Water Science and Technology, 25, 157-157 (1992)
Bacterial Competition Assay Based on Extracellular D-amino Acid Production
Alvarez, Laura and Cava, Felipe
The ISME Journal, 2018(3), 249-259 (2017)
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Questions

1–2 of 2 Questions  

  1. can we use this as an anterograde tracers to detect neuronal terminations

    1 answer

    1. This product has not been tested as an anterograde tracer to detect neuronal terminations, and no reference was found for this type of application. The products C3741 Cholera Toxin B subunit and L8777 Lectin from Phytolacca americana (pokeweed) had been referenced as neuroanatomical tracers. Below are the references:

      "Unmyelinated fibers of the anterior ethmoidal nerve in the rat co-localize with neurons in the medullary dorsal horn and ventrolateral medulla activated by nasal stimulation" by Hollandsworth MP, et al., Brain Research (2009).

      "An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: immunohistochemical localization of an axonally transported plant lectin, Phaseolus vulgaris leucoagglutinin (PHA-L)" by C R Gerfen, P E Sawchenko, Brain Research (1984-01-09).

      Helpful?

  2. What is the difference between different types of peroxidase? (for instance Type I, Type II, Type IIA)

    1 answer

    1. The type designations for these enzymes are in-house designations and do not represent any kind of biochemical significance.

      Helpful?

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