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D5545

DL-Ditiotreitol

BioXtra, ≥99.0% (titration)

Sinónimos:

treo-1,4-dimercapto-2,3-butanediol, DTT, Reactivo de Cleland

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Tamaño de envaseSKUDisponibilidadPrecio
1 g
Comprobar disponibilidad del carrito
$104.000
5 g
Comprobar disponibilidad del carrito
$333.000
25 g
Comprobar disponibilidad del carrito
$1.180.000

Acerca de este artículo

Fórmula lineal:
HSCH2CH(OH)CH(OH)CH2SH
Número CAS:
Peso molecular:
154.25
UNSPSC Code:
12352201
NACRES:
NA.21
PubChem Substance ID:
EC Number:
222-468-7
Beilstein/REAXYS Number:
1719757
MDL number:

$104.000


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product line

BioXtra

Quality Segment

assay

≥99.0% (titration)

form

powder

reaction suitability

reagent type: reductant

impurities

Insoluble matter, passes filter test, ≤0.3% 4,5-dihydroxy-1,2-dithiane

pH

4.0-6.0 (20-25 °C, 0.1 m in H2O)

mp

41-44 °C (lit.)

solubility

H2O: 0.1 M, clear, colorless

anion traces

sulfate (SO42-): ≤0.005%

cation traces

Al: ≤0.0005%, As: ≤0.0001%, Ba: ≤0.0005%, Bi: ≤0.0005%, Ca: ≤0.001%, Cd: ≤0.0005%, Co: ≤0.0005%, Cr: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Li: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0005%, Mo: ≤0.0005%, Na: ≤0.1%, Ni: ≤0.0005%, Pb: ≤0.0005%, Sr: ≤0.0005%, Zn: ≤0.0005%

absorption

≤0.100 at 280 in H2O at 0.1 M, ≤0.400 at 260 in H2O at 0.1 M

storage temp.

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

Application

Un reactivo excelente para el mantenimiento de los grupos SH en estado reducido; reduce cuantitativamente los disulfuros. El DTT reduce de manera eficaz los enlaces disulfuro de las proteínas en tampones de muestra antes de la SDS-PAGE. El DTT puede utilizarse también para reducir el puente disulfuro del reticulante N,N′-bis(acriloil)cistamina con el fin de separar la matriz de un gel de poliacrilamida. El DTT es menos mordaz y menos tóxico que el 2-mercaptoetanol. Normalmente se requiere una concentración de DTT siete veces inferior (100 mM) a la concentración utilizada del 2-mercaptoetanol (5 % v/v, 700 mM).

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1 of 1

Este artículo
D9779D916343815
assay

≥99.0% (titration)

assay

≥98% (HPLC), ≥99% (titration)

assay

≥99%

assay

≥99.5% (RT)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

product line

BioXtra

product line

-

product line

-

product line

BioUltra

anion traces

sulfate (SO42-): ≤0.005%

anion traces

-

anion traces

-

anion traces

sulfate (SO42-): ≤50 mg/kg

mp

41-44 °C (lit.)

mp

41-44 °C (lit.)

mp

40-44 °C, 41-44 °C (lit.)

mp

41-44 °C (lit.)

form

powder

form

powder

form

powder

form

powder


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pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Protocolos

SEC-MS protocol for rapid glycoprofiling of monoclonal antibodies with antibody purification and mass spectrometer calibration.

A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.

Artículos

A RPLC-MS workflow for mass analysis of reduced monoclonal antibody, trastuzumab, including reduction procedures, calibration, and system suitability tests.

Workflows for monoclonal antibody adalimumab characterization ensure drug safety and efficacy through critical quality attribute analysis.

A RPLC-MS workflow for mass analysis of non-reduced monoclonal antibody, trastuzumab, including calibration, and system suitability tests.

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Número de artículo de comercio global

SKUGTIN
D5545-25G04061835544011
D5545-5G04061835515981
D5545-1G04061833586419

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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