C2780

Chondroitinase AC aus Flavobacterium heparinum

Name: Chondroitinase AC aus <I>Flavobacterium heparinum</I>
Brand: SIGMA
Price: 832 EUR

lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

Synonym(e):

Chondroitin-AC-Lyase

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Über diesen Artikel

CAS-Nummer:

9047-57-8

UNSPSC Code:

12352204

NACRES:

NA.54

EG-Nummer:

4.2.2.5 (BRENDA, IUBMB)

MDL number:

MFCD00130796

Specific activity:

0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

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Unterstützung erhalten

conjugate

(Glucosaminoglycan)

Quality Level

200

form

lyophilized powder

specific activity

0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

composition

Protein, ~15% Lowry

solubility

0.02 M phosphate buffer: soluble (pH 7.0)

foreign activity

Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount

storage temp.

−20°C

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Dieser Artikel	C0954	E2039	C3667
Sigma-Aldrich C2780 Chondroitinase AC from Flavobacterium heparinum	Sigma-Aldrich C0954 Chondroitinase C from Flavobacterium heparinum	Sigma-Aldrich E2039 Chondroitinase AC from Flavobacterium heparinum	Sigma-Aldrich C3667 Chondroitinase ABC from Proteus vulgaris
specific activity 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)	specific activity ≥200 units/mg solid	specific activity ≥200 units/mg protein	specific activity 50-250 units/mg protein
form lyophilized powder	form lyophilized powder	form lyophilized solid	form lyophilized powder
solubility 0.02 M phosphate buffer: soluble (pH 7.0)	solubility -	solubility -	solubility 0.01% bovine serum albumin aqueous (BSA) solution: soluble
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 300
foreign activity Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount	foreign activity -	foreign activity -	foreign activity protease, essentially free

Application

Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.[1]

Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.

Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides

Biochem/physiol Actions

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

Preparation Note

Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.

Other Notes

Weitere Informationen über Enzyme für die Analyse komplexer Kohlenhydrate finden Sie unter www.sigma-aldrich.com/enzymeexplorer

Contains potassium phosphate buffer salts and BSA as stabilizer.

One unit will cause a ΔA₂₃₂ of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).

used together

Produkt-Nr.

Beschreibung

Preisangaben

E2039

Chondroitinase AC from Flavobacterium heparinum, recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

0000476610

0000386545

0000384938

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Global Trade Item Number

SKU	GTIN
C2780-5UN	04061833484609

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