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T3449

TrypZean® Solution, 1×

recombinant, expressed in corn, sterile-filtered

Sinónimos:

Trypsin

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A ustedes/SKUDisponibilidadPrecio
100 mL
Comprobar disponibilidad del carrito
$50.600
500 mL
Comprobar disponibilidad del carrito
$200.000

Acerca de este artículo

NACRES:
NA.75
UNSPSC Code:
12352204
Recombinant:
expressed in corn
Concentration:
1 ×

$50.600

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recombinant

expressed in corn

Quality Segment

300

sterility

sterile-filtered

form

aqueous solution

concentration

1 ×

technique(s)

cell culture | mammalian: suitable

pH

7.70-8.30

shipped in

dry ice

storage temp.

−20°C

General description

TrypZean® solution, 1× is formulated with TrypZean, recombinant bovine trypsin expressed in corn and manufactured utilizing ProdiGene′s proprietary transgenic plant protein expression system. This product is optimized for cell dissociation in both serum-free and serum-supplemented adherent cell cultures.

Application

TrypZean® Solution, 1× has been used:

  • for the suspension of pellet during DNA extraction[1]
  • digestion of cells to single-cell suspension for pharmacotyping of 2D cells[2]
  • as recombinant trypsin to induce cell division of Vero cells sub cultivated in IPT-AF medium[3]

Features and Benefits

TrypZean® Solution provides the following features and benefits:
  • Animal component-free – eliminates the risk of viruses, BSE, or other potential adventitious agents
  • TrypZean is recombinant trypsin – using the same enzyme (with the same kinetics) for cell detachment means minimal protocol changes
  • Enzyme inhibition – soybean trypsin inhibitor and other inhibitors work the same with TrypZean as they do with native trypsins (weight-to-weight basis)
  • High purity – TrypZean provides increased specificity and eliminates contaminating activities found in lower purity enzymes
  • Convenience – TrypZean solution is a sterile-filtered solution formulated at the optimal concentration to dissociate adherent cell lines

Legal Information

Trypzean is a registered trademark of ProdiGene, Inc.

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Este artículo
T2601C5789C5914
TrypZean® Solution, 1× recombinant, expressed in corn, sterile-filtered

Sigma-Aldrich

T3449

TrypZean® Solution, 1×

Disolución de tripsina-EDTA 1X, sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

Sigma-Aldrich

T2601

Disolución de tripsina-EDTA

Cell Dissociation Solution Non-enzymatic 1x Prepared in Hanks′ Balanced Salt Solution without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

C5789

Cell Dissociation Solution Non-enzymatic 1x

Cell Dissociation Solution Non-enzymatic 1x Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

C5914

Cell Dissociation Solution Non-enzymatic 1x

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

recombinant

expressed in corn

recombinant

-

recombinant

-

recombinant

-

form

aqueous solution

form

-

form

solution

form

liquid

concentration

1 ×

concentration

1X

concentration

1 ×

concentration

1 ×

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

  1. What is the working concentration of recombinant trypsin used ?

    1 answer

    1. Product T3449 is prepared from a 0.0025% concentration.

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