No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.
C5914
Cell Dissociation Solution Non-enzymatic 1x
Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture
Sinónimos:
Dissociation Solution
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 100 mL | Comprobar disponibilidad del carrito | $53.900 |
Acerca de este artículo
Concentration:
1 ×
Servicio técnico
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Permítanos ayudarleQuality Level
sterility
sterile-filtered
product line
BioReagent
form
liquid
concentration
1 ×
technique(s)
cell culture | mammalian: suitable
pH
6.9-7.1
shipped in
wet ice
storage temp.
2-8°C
General description
Cell dissociation by non-enzymatic methods in human mesenchymal stromal cells aid in their detachment and preserves their functionality especially the expression of cell surface chemokine receptor.
Application
Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.
Cell Dissociation Solution Non-enzymatic 1x has been used for the dissociation of retinal astrocytes culture, U87 glioblastoma tumor cells and cardiac stem cells (CSCs).
1 of 1
Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable |
| form liquid | form solution | form aqueous solution | form liquid |
| concentration 1 × | concentration 1 × | concentration 1 × | concentration - |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility - |
| storage temp. 2-8°C | storage temp. room temp | storage temp. −20°C | storage temp. - |
| pH 6.9-7.1 | pH 6.5-7.5 | pH 7.70-8.30 | pH - |
flash_point_f
Not applicable
flash_point_c
Not applicable
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 2
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Ineke D C Jansen et al.
International journal of molecular sciences, 21(17) (2020-08-29)
Lysosome associated membrane proteins (LAMPs) are involved in several processes, among which is fusion of lysosomes with phagosomes. For the formation of multinucleated osteoclasts, the interaction between receptor activator of nuclear kappa β (RANK) and its ligand RANKL is essential.
Isolation and characterization of murine retinal astrocytes
Scheef E, et al.
Molecular Vision, 11(4), 613-624 (2005)
Non-enzymatic dissociation of human mesenchymal stromal cells improves chemokine-dependent migration and maintains immunosuppressive function
Garg A, et al.
Cytotherapy, 16(4), 545-559 (2014)
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| C5914-100ML | 04061833502662 |
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When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?
1 answer-
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Can one use Product C5914, Cell Dissociation Solution, with tissue?
1 answer-
We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.
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Does Product C5914, Cell Dissociation Solution, work with epithelial cells?
1 answer-
It will work with most cell types.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does Product C5914, Cell Dissociation Solution, contain EDTA?
1 answer-
Yes, it does contain EDTA; the concentration is proprietary.
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