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T1706

Sigma-Aldrich

TransIT-CRISPR®

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.51

aplicaciones

CRISPR

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Descripción general

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Características y beneficios

  • Highly efficient, non-liposomal polymeric transfection/delivery method for use with MISSION CRISPR/Cas9 DNA, RNA, Cas9 RNP Complexes and siRNA.
  • TransIT-CRISPR is a simple, fast, effective, and versatile. One transfection reagent to deliver CRISPR-Cas9 components in a variety of formats.
  • Effective in most cell lines including difficult-to-transfect cells.

Componentes

0.4ml and 1ml DNA and siRNA non-liposomal, polymeric transfection reagent

Ligadura / enlace

To order MISSION® CRISPR reagents, please click here

For more information and transfection protocols, please visit SigmaAldrich.com/TransITCRISPR

Otras notas

  • TransIT-CRISPR has been tested in variety of cell types including U20S and HEK 293, A549 and HeLa among others.
  • It is recommended to transfect MISSION CRISPR DNA and RNA with the TransIT-CRISPR transfection reagent.
  • Sigma-Aldrich® is the exclusive distributor of TransIT-CRISPR from Mirus Bio LLC.

Información legal

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMA-ALDRICH is a registered trademark of Merck KGaA, Darmstadt, Germany
TransIT-CRISPR is a registered trademark of Mirus Bio LLC

Pictogramas

FlameExclamation mark
GHS02,GHS07

Palabra de señalización

Danger

Frases de peligro

H225,H319

Clasificaciones de peligro

Eye Irrit. 2 - Flam. Liq. 2

Código de clase de almacenamiento

3 - Flammable liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

57.2 °F - closed cup

Punto de inflamabilidad (°C)

14.0 °C - closed cup

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
71113920
51053481
51053795
SLBP2593V
SLBP2596V

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Visite la Librería de documentos

Aitziber Buqué et al.
Nature communications, 11(1), 3819-3819 (2020-08-01)
Hormone receptor (HR)+ breast cancer (BC) causes most BC-related deaths, calling for improved therapeutic approaches. Despite expectations, immune checkpoint blockers (ICBs) are poorly active in patients with HR+ BC, in part reflecting the lack of preclinical models that recapitulate disease

Protocolos

PURedit™ CRISPR Synthetic Guide RNAs and Cas9 Protein

Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity

Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Contenido relacionado

CRISPR Cas9 Proteins

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CRISPR Cas9 Ribonucleoprotein (RNP) Application Note

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

Gene Expression & Silencing

Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).

Expresión y silenciamiento génicos

Sigma-Aldrich® Advanced Genomics es el principal proveedor de tecnologías de edición y silenciamiento de genes, entre ellas, CRISPR, Cas9, ARN guía sintético (sgRNA) y nucleasa con dedos de zinc (ZFN).

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico