Name: CRISPR/Cas9 Products and Services
Brand: SIGMA

<span style="color: #0e69af;">.</span><br>Order Custom<br>gRNAs<br><span style="color: #0e69af;">.</span>

<span style="color: #0e69af;">.</span><br>Design<br>CRISPR<br><span style="color: #0e69af;">.</span>

Order<br>*Guaranteed<br>Predesigned<br>gRNA

CRISPR Compatible Products

<h3>Guide RNAs</h3>
<p>Visit <a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispr-clone-control-and-cas9-essentials" target="_blank">CRISPR Essentials</a> to review CRISPR formats and products that best fit your research needs.</p>
<p>Order Guaranteed Predesigned CRISPR gRNAs</p>
<ul>
<li><a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispr-clone-control-and-cas9-essentials" target="_blank">Synthetic SygRNA<sup>®</sup> gRNAs</a> available in two-part (<a href="/US/en/search/tracr?focus=products&amp;page=1&amp;perPage=30&amp;sort=relevance&amp;term=tracr&amp;type=product" target="_blank">crRNA:tracrRNA</a>) or one-part <a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispr-clone-control-and-cas9-essentials" target="_blank">sgRNA</a> formats. </li>
<li>Perfect for animal model generation and cell model engineering.</li>
<li>Recommended to complex with Cas9 protein.</li>
<li>Lentivirus gRNAs are available as ready-to-use lentivirus transduction particles. </li>
<li>Perfect for difficult-to-transfect or primary cells.</li>
<li>Plasmid DNA transfection-grade plasmid gRNAs available in a variety of vectors.</li>
<li>Perfect for transfection, nucleofection, and lentiviral packaging (Note: lentiviral packaging can only be achieved by selecting a lentiviral vector).</li>
<li><i>In Vitro </i>Transcribed RNA  is provided in DNase-, RNase-, and protease-free molecular-grade water.</li>
</ul>
<p>Design CRISPR</p>
<ul>
<li>Click on the CRISPR Design Tool to get started.</li>
<li>Select your desired genome, choose your gene of interest, and set parameters for the designs.</li>
<li>View and export a list of ranked gRNAs.</li>
</ul>
<p>Order Your CRISPR  gRNAs</p>
<ul>
<li>Click on the Order gRNAs button and upload your gRNA sequences.</li>
<li>Choose the format: Synthetic gRNA, Transfection-grade plasmid DNA, Lentiviral transduction particles, or IVT gRNA.</li>
<li>Customize the vector and configuration.</li>
<li><a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispr-clone-control-and-cas9-essentials" target="_blank">Visit the CRISPR Essentials page to compare options.</a></li>
<li>Purchase clone quantities to receive additional discounts.</li>
</ul>
<p><i>*</i>We are so confident in the performance of our SygRNA<sup>®</sup> products, that we fully guarantee the quality and performance of any guide we produce, including custom sequences. If your SygRNA<sup>®</sup> guides do not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge. To qualify for this guarantee, please send an image or sequencing data from a single experiment demonstrating detectable cleavage using one of our positive controls, side-by-side with the negative results from your SygRNA<sup>®</sup> guide.To receive your replacement, simply email us here and include sample data from a representative experiment (T7E1, TIDE, or NGS)<i>.</i></p>


<h3>Cas9 Products</h3>
<ul>
<li><a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispr-clone-control-and-cas9-essentials" target="_blank">Proteins</a> — Choose from the largest portfolio of Cas9 proteins available for any application.</li>
<li><a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispr-clone-control-and-cas9-essentials" target="_blank">Plasmid DNA</a> — Cas9 expression plasmids utilize the CMV promoter for effective, transient expression. Available with and without fluorophore and antibiotic selection elements.</li>
<li><a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispr-clone-control-and-cas9-essentials" target="_blank">Lentivirus</a> — Select Cas9-expressing lentivirus transduction particles, with blasticidin or neomycin resistance.</li>
<li><a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispr-clone-control-and-cas9-essentials">CRISPR Modulation</a> – CRISPR activation and inhibition (CRISPRa/CRISPRi) utilize catalytically dead (dCas9) to allow gain and loss of function studies to complement your knockout research.</li>
</ul>


<h3>Controls and Companion Products </h3>
<ul>
<li><a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispr-clone-control-and-cas9-essentials">Controls</a> — Positive and negative controls in plasmid and lentiviral formats, available in a variety of vector options.</li>
<li><a href="/US/en/search/tracr?focus=products&amp;page=1&amp;perPage=30&amp;sort=relevance&amp;term=tracr&amp;type=product" target="_blank">TracrRNA</a> — Chemically modified and unmodified versions required to pair with crRNA.</li>
<li><a href="/search/CRISPR+transfection+reagent?focus=products&amp;page=1&amp;perPage=30&amp;sort=relevance&amp;term=CRISPR+transfection+reagent&amp;type=product" target="_blank">Transfection Reagents</a> — Transfection/delivery products for use with Sigma-Aldrich<sup>®</sup> plasmid DNA, Synthetic RNA, and Cas9 Ribonucleoprotein (RNP) Complexes.</li>
<li><a href="/US/en/product/sigma/shp002" target="_blank">Lentiviral Packaging Mix</a> — Recommended for packaging Lenti-CRISPR plasmid DNA into pseudo-typed lentiviral particles for downstream transduction applications. </li>
<li><a href="/US/en/product/sigma/hsoligoint" target="_blank">CRISPR Integration Kit</a> — Includes a validated CRISPR that cleaves DNA with high efficiency and a  standardized oligo donor designed to survey homology dependent repair frequencies in a wide variety of cell lines.</li>
</ul>


<h3>CRISPR Screening Tools: Lentiviral Libraries, Collections, and Panels</h3>
<h4>Sanger Whole Genome Knockout (KO) Arrayed CRISPR Libraries — R&amp;D 100 Award Winner</h4>
<p>Your solution for screening the human and mouse whole genomes for drug discovery or target identification.</p>
<ul>
<li>Over 74,000 individual off-the-shelf CRISPR gRNAs designed to maximize KO of every protein-coding gene in human and mouse genomes.</li>
<li>Available as individual clones, gene panels, and whole genome libraries.</li>
<li><a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/sanger-crispr-arrayed-library" target="_blank">Learn more about Sanger Arrayed CRISPR Libraries.</a></li>
</ul>
<h4>Sanger QuickPick™ KO gRNAs</h4>
<p>Order off-the-shelf KO gRNAs from the award-winning Sanger Whole Genome KO Arrayed CRISPR Libraries.</p>
<ul>
<li>A fast, cost-efficient way to quickly knock out your gene of interest.</li>
<li>Available formats:<ul>
<li>Bacteria Glycerol Stocks</li>
<li>Plasmid DNA</li>
<li>Lentiviral transduction particles</li>
</ul>
</li>
</ul>
<h4>Whole Genome KO Pooled Libraries</h4>
<ul>
<li>GeCKO v2 CRISPR Knockout Pooled Libraries</li>
<li>Available for human and mouse genomes<ul>
<li>Vector options: lentiCRISPRv2 (Cas9+gRNA on same vector); lentiGuide-Puro (gRNA-only vector)</li>
<li>Whole Genome Lentiviral CRISPR Pool</li>
</ul>
</li>
</ul>
<h4>Pooled CRISPR Screening with 10x Genomics Compatibility</h4>
<ul>
<li><a href="/US/en/technical-documents/technical-article/genomics/functional-genomics-screening/discovery-at-single-cell-resolution">Custom CRISPR lentiviral pools</a> built to any specifications with 10x Genomics compatibility for analysis at single-cell resolution</li>
<li>Perfect for honing in on candidates after whole-genome screening. Our optimized lentiviral vectors allow tracking of both gene expression and gene perturbation in the same assay for functional phenotype characterization</li>
</ul>
<h4><a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispri-crispra-pooled-lentiviral-screening" target="_blank">Whole Genome Activator Libraries</a></h4>
<p>Synergistic Activation Mediators (SAM) are potent transcriptional activation protein complexes. The SAM CRISPRa system uses CRISPR-Cas9-mediated guidance to target SAM components to gene promoters, enabling site-specific transcriptional activation of a gene of interest.</p>
<ul>
<li><a href="/US/en/product/sigma/hsampuro">Human Puro Kit</a></li>
<li><a href="/US/en/product/sigma/hsamzeo">Human Zeo Kit</a></li>
<li><a href="/US/en/product/sigma/msampuro">Mouse Puro Kit</a></li>
<li><a href="/US/en/product/sigma/msamzeo">Mouse Zeo Kit</a></li>
</ul>
<p><a href="/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/deconvolution-of-pooled-shrna-screens" target="_blank">Deconvolution Services</a></p>


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10x Genomics compatible CRISPR pools

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Sigma-Aldrich

CRISPR/Cas9 Products and Services Design and order CRISPR gRNA, Cas9, screening libraries, controls and companion products. Formats include plant, lentivirus, IVT-RNA, plasmid, synthetic, and protein.; Synonyms: CRISPR,CRISPRs,Cas9,Crispr RNA,Crispr/Cas System at Sigma-Aldrich

CRISPR/Cas9 Products and Services Design and order CRISPR gRNA, Cas9, screening libraries, controls and companion products. Formats include plant, lentivirus, IVT-RNA, plasmid, synthetic, and protein. | Sigma-Aldrich

Design and order CRISPR gRNA, Cas9, screening libraries, controls and companion products. Formats include plant, lentivirus, IVT-RNA, plasmid, synthetic, and protein.

CRISPR/Cas9 Products and Services Design and order CRISPR gRNA, Cas9, screening libraries, controls and companion products. Formats include plant, lentivirus, IVT-RNA, plasmid, synthetic, and protein.

CRISPR/Cas9 Products and Services

Synonym: CRISPR, CRISPRs, Cas9, Crispr RNA, Crispr/Cas System, Synthetic CRISPR, crRNA, tracrRNA

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CRISPR/Cas9 Products and Services

Design and order CRISPR gRNA, Cas9, screening libraries, controls and companion products. Formats include plant, lentivirus, IVT-RNA, plasmid, synthetic, and protein.

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