This endoproteinase is not sterile and has not been tested for suitability in cell culture. It is recommended for use in protein sequencing assays.
P6181
Glu-C Protease
Cleaves at glutamic acid residues, suitable for Mass Spectrometry, from Staphylococcus aureus V8
Sinónimos:
V8 Protease
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
200-350-6
MDL number:
eCl@ss:
32160410
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Servicio técnico
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Permítanos ayudarleNombre del producto
Endoproteinase Glu-C from Staphylococcus aureus V8, suitable for protein sequencing, lyophilized powder
grade
Proteomics Grade
Quality Level
form
lyophilized powder
analyte chemical class(es)
amino acids
suitability
suitable for protein sequencing
storage temp.
−20°C
Application
Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for:
- obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies.[1]
- the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination.
- limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase).
- the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.
Biochem/physiol Actions
Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine endoprotease, which hydrolyzes peptide bonds at the carboxyl side of glutamyl and aspartyl residues. The specificity of Glu-C is dependent upon the buffer and pH employed as well as the structure around the potential cleavage site. In ammonium acetate (pH 4.0) or ammonium bicarbonate (pH 7.8), the enzyme preferentially cleaves glutamyl bonds; whereas, in phosphate buffer (pH 7.8) Glu-C will cleave at either site. Glu-C is reported to be active in the presence of 0.2% SDS (sodium dodecyl sulfate) and in 4.0M urea.
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Este artículo | |||
|---|---|---|---|
| grade Proteomics Grade | grade - | grade Proteomics Grade | grade Proteomics Grade |
| suitability suitable for protein sequencing | suitability - | suitability - | suitability suitable for protein sequencing |
| form lyophilized powder | form lyophilized powder | form lyophilized | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| analyte chemical class(es) amino acids | analyte chemical class(es) - | analyte chemical class(es) - | analyte chemical class(es) amino acids |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Instructions
M Jarosch et al.
Microbiology (Reading, England), 146 ( Pt 2), 273-281 (2000-03-09)
The cell surface of Bacillus stearothermophilus ATCC 12980 is completely covered with an oblique S-layer lattice. To investigate sequence identities and a common structure-function relationship in S-layer proteins of different B. stearothermophilus wild-type strains, the nucleotide sequence encoding the S-layer
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| P6181-50UG | 04061835574698 |
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Hi, is this enzyme sterile and suitable for cell culture? Thank you.
1 answer-
Helpful?
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Hi, I was just wondering you can give me the amino acid sequence for this protein. I can't find it on the page.
1 answer-
The product does not need to be sequenced to get released. Unfortunately there is no in-house information about product sequence.
Helpful?
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