This product is not tested for solubility. However, other Protamine sulfate products currently available are dissolved initially in 6N Hydrochloric acid at 25 mg/mL. This stock solution may then be diluted out with aqueous buffer or medium. In addition, a brief search of the literature reveals that this material may also be prepared by dissolving in HPLC grade water to a concentration of 10mg/ml. A stock solution was diluted to a final working concentration in growth media of 10?g/ml and stored at -20°C. This information has not been validated.
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http://eprints.nottingham.ac.uk/11280/1/PhD_Thesis.pdf
P3369
Protamine sulfate
solid, 90.0-110.0% dry basis,≥92% (chromatography), suitable for transduction
Sinónimos:
Salmine sulfate from salmon sperm, Protamine sulfate
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 10 g | Comprobar disponibilidad del carrito | $216.000 | |
| 100 g | Comprobar disponibilidad del carrito | $1.310.000 |
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Servicio técnico
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Permítanos ayudarleNombre del producto
Protamine sulfate, meets USP testing specifications
agency
meets USP testing specifications
Quality Level
assay
≥92% (chromatography), 90.0-110.0% dry basis
form
solid
mol wt
Mr 5000-10000
technique(s)
transduction: suitable
shipped in
ambient
storage temp.
2-8°C
General description
Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.
Application
Protamine sulfate has been used in the transduction of
- lentiviruses into human embryonic 293T cells[1]
- shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
- lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells
Biochem/physiol Actions
Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.
Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.
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Este artículo | |||
|---|---|---|---|
| assay ≥92% (chromatography), 90.0-110.0% dry basis | assay ≥98% (HPLC) | assay - | assay - |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level 300 |
| form solid | form powder | form - | form solid |
| shipped in ambient | shipped in ambient | shipped in - | shipped in - |
| storage temp. 2-8°C | storage temp. 15-25°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt Mr 5000-10000 | mol wt - | mol wt - | mol wt - |
Clase de almacenamiento
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Moosung Lee et al.
eLife, 9 (2020-12-18)
The immunological synapse (IS) is a cell-cell junction between a T cell and a professional antigen-presenting cell. Since the IS formation is a critical step for the initiation of an antigen-specific immune response, various live-cell imaging techniques, most of which
Meagan R Rollins et al.
Current protocols in immunology, 129(1), e97-e97 (2020-05-21)
T lymphocytes are capable of specific recognition and elimination of target cells. Physiological antigen recognition is mediated by the T cell receptor (TCR), which is an alpha beta heterodimer comprising the products of randomly rearranged V, D, and J genes.
Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCs
Hematopoietic Differentiation of Human Pluripotent Stem Cells, 91-101 (2015)
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| P3369-100G | 04061835558292 |
| P3369-10G | 04061835558308 |
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In what solution can this particular protamine sulfate be dissolved? Sterile water, PBS, DMSO,...?
1 answer-
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