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M0644

Medio esencial mínimo Eagle

Name: Medio esencial mínimo Eagle
Brand: SIGMA
Price: 135000 CLP

Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

MEM

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Tamaño de envase	SKU	Disponibilidad	Precio
10 L		Comprobar disponibilidad del carrito	$135.000
10 x 1 L		Comprobar disponibilidad del carrito	$141.000

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NACRES:

NA.75

UNSPSC Code:

12352207

$135.000

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Propiedades

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO₃: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

Descripción

General description

Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

Application

Minimum essential medium Eagle has been used to:

maintain natural killer (NK) cell lines[1]
culture Leishmania donovani strains[2]
culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles[3]

Preparation Note

Formulated to contain 10.1 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

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Este artículo	M5650	M4526	M2279
Sigma-Aldrich M0644 Minimum Essential Medium Eagle	Sigma-Aldrich M5650 Minimum Essential Medium Eagle	Sigma-Aldrich M4526 Minimum Essential Medium Eagle	Sigma-Aldrich M2279 Minimum Essential Medium Eagle
form powder	form liquid	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components NaHCO₃: no L-glutamine: yes phenol red: yes HEPES: no sodium pyruvate: yes	components phenol red: yes NaHCO₃: yes L-glutamine: no sodium pyruvate: no HEPES: no Earle’s salts (5% CO₂): yes	components Earle’s salts (5% CO₂): yes sodium pyruvate: yes glucose: yes L-glutamine: no HEPES: no Hanks’ salts (2% CO₂): no phenol red: yes NaHCO₃: yes stable glutamine: no	components phenol red: yes sodium pyruvate: no L-glutamine: no HEPES: no NaHCO₃: yes Earle’s salts (5% CO₂): yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
Quality Level 500	Quality Level 400	Quality Level 400	Quality Level 400

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Artículos

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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Número de artículo de comercio global

SKU	GTIN
M0644-10X1L	04061833993798
M0644-10L	04061833993781

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