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M4526

Sigma-Aldrich

Medio esencial mínimo Eagle

Minimum Essential Medium Eagle

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

αMEM, MEM

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500 ML
$63.300
6 X 500 ML
$356.000
24 X 500 ML
$1.170.000

$63.300

Fecha estimada de envío23 de abril de 2025

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500 ML
$63.300
6 X 500 ML
$356.000
24 X 500 ML
$1.170.000

About This Item

Código UNSPSC:
12352207
NACRES:
NA.71

$63.300

Fecha estimada de envío23 de abril de 2025

Solicitar un pedido a granel

Nombre del producto

Medio esencial mínimo Eagle, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Nivel de calidad

400

esterilidad

sterile-filtered

Formulario

liquid

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.
This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Aplicación

Minimum Essential Medium Eagle has been used to maintain:
  • immature cumulus-oocyte complexes from mice[1]
  • human mesenchymal stem cells (MSC)[2]
  • mouse pre-osteoblastic cell line[3]

Reconstitución

Supplement with 0.292 g/L L-glutamine.

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suplemento

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también adquirido normalmente con este producto

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
RNBM0562
RNBL7706
RNBL2659
RNBL5431
RNBL0707

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Michelle L Churchman et al.
Cancer cell, 33(5), 937-948 (2018-04-24)
Somatic genetic alterations of IKZF1, which encodes the lymphoid transcription factor IKAROS, are common in high-risk B-progenitor acute lymphoblastic leukemia (ALL) and are associated with poor prognosis. Such alterations result in the acquisition of stem cell-like features, overexpression of adhesion
Zhourui Ma et al.
Journal of molecular histology, 51(3), 241-250 (2020-05-14)
Using a large-scale quantitative mesenchymal stem cells (MSCs) membrane proteomics analysis, we identified a large group of ciliary proteins in the MSCs membrane fraction, which prompted us to examine the cilia, intricate organelles that were originally discovered approximately 100 years ago.
Jason M Lajoie et al.
Protein engineering, design & selection : PEDS, 32(5), 219-230 (2019-11-27)
Yeast display immunoprecipitation is a combinatorial library screening platform for the discovery and engineering of antibodies against membrane proteins using detergent-solubilized membrane fractions or cell lysates as antigen sources. Here, we present the extension of this method for the screening
Philipp von Eisenhart-Rothe et al.
Investigative ophthalmology & visual science, 59(12), 5082-5097 (2018-10-30)
Vision loss caused by photoreceptor death represents one of the first symptoms in neuronal ceroid lipofuscinosis, a condition characterized by accumulation of intracellular waste. Cln6nclf mice have a naturally occurring mutation in ceroid-lipofuscinosis neuronal (CLN) protein 6 and are a
Ricardo Rodrigues-Pinto et al.
Scientific reports, 8(1), 12866-12866 (2018-08-29)
The adult nucleus pulposus originates from the embryonic notochord, but loss of notochordal cells with skeletal maturity in humans is thought to contribute to the onset of intervertebral disc degeneration. Thus, defining the phenotype of human embryonic/fetal notochordal cells is
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MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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