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M4526

Medio esencial mínimo Eagle

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Minimum Essential Medium Eagle

Sinónimos:

αMEM, MEM

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A ustedes/SKUDisponibilidadPrecio
500 mL
Comprobar disponibilidad del carrito
$65.600
6 x 500 mL
Comprobar disponibilidad del carrito
$369.000
24 x 500 mL
Comprobar disponibilidad del carrito
$1.210.000

Acerca de este artículo

UNSPSC Code:
12352207
NACRES:
NA.71

$65.600

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Nombre del producto

Medio esencial mínimo Eagle, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.
This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Application

Minimum Essential Medium Eagle has been used to maintain:
  • immature cumulus-oocyte complexes from mice[1]
  • human mesenchymal stem cells (MSC)[2]
  • mouse pre-osteoblastic cell line[3]

Preparation Note

Supplement with 0.292 g/L L-glutamine.

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Este artículo
M8042M5650M0644
Medio esencial mínimo Eagle Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M4526

Medio esencial mínimo Eagle

Medio esencial mínimo Eagle Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M8042

Medio esencial mínimo Eagle

Medio esencial mínimo Eagle With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M5650

Medio esencial mínimo Eagle

Sigma-Aldrich

Sigma-Aldrich

M0644

Medio esencial mínimo Eagle

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

powder

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

components

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

components

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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