The formulation for L3022 has not changed over the years. The "low salt" label was added later to help customers identify the formulation more easily.
L3022
Caldo LB (Lennox)
Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.
LB Broth (Lennox)
Sinónimos:
Caldo Lennox, Caldo Luria Bertani
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 250 g | Comprobar disponibilidad del carrito | $40.400 | |
| 1 kg | Disponible para enviar HOYdesdePUDAHUEL | $163.000 | |
| 6 x 1 kg | Comprobar disponibilidad del carrito | $954.000 |
Acerca de este artículo
NACRES:
NA.85
UNSPSC Code:
41106200
Servicio técnico
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Molecular Biology
Quality Segment
sterility
non-sterile
form
powder
composition
NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(2% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Lennox LB es un medio para crecimiento microbiano muy citado que se utiliza para el cultivo de E. coli. Este caldo microbiano rico en nutrientes contiene péptidos, aminoácidos y carbohidratos en una formulación baja en sales.
Application
El caldo Luria se utiliza para el mantenimiento y la propagación de Escherichia coli. E. coli crece más deprisa en el caldo Luria porque la triptona y la levadura proporcionan factores de crecimiento esenciales que, de otro modo, E. coli tendría que sintetizar. El caldo Luria contiene también electrólitos esenciales para el transporte y el equilibrio osmóticos, debido al componente NaCl.
Se utilizó para la resuspensión de fracciones celulares de T. pyriformis en un estudio de fraccionamiento de extractos celulares de Trimastix.[1]
Se utilizó para la resuspensión de fracciones celulares de T. pyriformis en un estudio de fraccionamiento de extractos celulares de Trimastix.[1]
Idóneo para cultivo no selectivo de cepas de E. coli para clonación, producción de plásmidos ADN y producción de proteínas recombinantes. También es adecuado para el cultivo selectivo cuando se añaden los antibióticos apropiados, entre ellos los que requieren bajas condiciones salinas, como la zeocina®.
Features and Benefits
El polvo Lennox LB proporciona:
- Un cómodo envase pequeño para no tener que pesar
- Fácil escalado utilizando tamaños de envase mayores
- Una alternativa económica al líquido
- Una formulación estándar
Preparation Note
1. Suspenda 20 g en 1 l de agua destilada.
2. Esterilice en autoclave durante 15 minutos a 121 ºC.
Para preparar el caldo Lennox L: Añada 1 g de glucosa y siga las instrucciones de preparación anteriores.
Para preparar el medio de Enquist y Sternberg: Agregue asépticamente 10 ml de sulfato de magnesio 1 M estéril después de haberlo esterilizado en autoclave.
2. Esterilice en autoclave durante 15 minutos a 121 ºC.
Para preparar el caldo Lennox L: Añada 1 g de glucosa y siga las instrucciones de preparación anteriores.
Para preparar el medio de Enquist y Sternberg: Agregue asépticamente 10 ml de sulfato de magnesio 1 M estéril después de haberlo esterilizado en autoclave.
Legal Information
Zeocin is a registered trademark of Cayla Sarl
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Este artículo | |||
|---|---|---|---|
| form powder | form powder | form tablet | form powder |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| composition NaCl, 5 g/L , Yeast Extract, 5 g/L , Tryptone, 10 g/L | composition - | composition - | composition Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) food and beverages | application(s) agriculture | application(s) food and beverages | application(s) food and beverages |
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Clase de almacenamiento
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocolos
General protocols for growth of competent cells in microbial medium.
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| L3022-250G | 04061835573431 |
| L3022-1KG | 04061835573424 |
| L3022-6X1KG | 04061835573448 |
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Are the new batches of the product labeled as "Low salt" consistent with the older batch that does not have the same label? Consistency in experiments is a concern.
1 answer-
Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Helpful?
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
Helpful?
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What is the difference among the many LB - Lennox products?
1 answer-
LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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