This product is assigned retest date of approximately 3 years from the date of Quality Release. This date is lot-specific and may be found in the product Certificate of Analysis. When stored at room temperature, in a tightly sealed container, the material will be stable at least until the assigned retest date.
L7275
LB Broth (Lennox)
LB Broth (Lennox)
Tablet microbial growth medium
Sinónimos:
Lennox broth , Luria Bertani Broth
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100 TABS
$148.000
500 TABS
$508.000
$148.000
Fecha estimada de envío30 de abril de 2025
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100 TABS
$148.000
500 TABS
$508.000
About This Item
Código UNSPSC:
12352200
NACRES:
NA.85
$148.000
Fecha estimada de envío30 de abril de 2025
Productos recomendados
grado
for molecular biology
Nivel de calidad
esterilidad
non-sterile
Formulario
tablet
técnicas
microbiological culture: suitable
aplicaciones
food and beverages
microbiology
temp. de almacenamiento
room temp
idoneidad
nonselective for Escherichia coli
nonselective for coliforms
Descripción general
Dehydrated Luria-Bertani (LB) broth is called the Lennox LB, which has amino acids as carbon source.[1]
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.
Aplicación
LB Broth (Lennox) has been used for culturing Escherichia coli[2][3] and bacterial artificial chromosomes (BACs).[4]
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Características y beneficios
Lennox LB tablets provide:
- Convenient small package to eliminate weighing
- Fast preparation – no stirring required
Componentes
10 g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents
Reconstitución
Add each 1.1g tablet in 48.3mL water (no stirring required). Autoclave for 20 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Información legal
Zeocin is a registered trademark of Cayla Sarl
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
Lot/Batch Number
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Protocolos
General protocols for growth of competent cells in microbial medium.
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How long can these tablets be stored at room temperature in the bottle (after opening).
1 answer-
Helpful?
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Do the tablets contain any additives to adjust the pH of the media? Also, what should the final pH of the media be?
1 answer-
The pH of the media can be 7.0, but depending on the application, it can range from 7.0 to 8.0. The pH can be adjusted using 1N NaOH and 1N HCl.
Helpful?
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Helpful?
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What is the difference among the many LB - Lennox products?
1 answer-
LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
Helpful?
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