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F2878

Ficoll® PM 70

Type 70

Ficoll® PM 70

Sinónimos:

Poly(sucrose-co-epichlorhydrin)

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Número CAS:
UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
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type

Type 70

Quality Level

form

powder

mol wt

~70,000

color

white

solubility

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

General description

Ficoll® PM70, a high molecular weight branched polysaccharide polymer, is believed that they do not contain any ionized groups. This sucrose-polymer is formed by the copolymerization of sucrose with epichlorhydrin.

Application

Ficoll® PM 70 has been used:
  • as a macromolecule in a comparative study to evaluate its effect in vitrification solutions
  • as a supplement in the vitrification solution for the vitrification procedure of oocytes
  • as a component of low serum medium for human lung fibroblast cell culture to promote a crowded environment

Biochem/physiol Actions

Ficoll is commonly used as a macromolecular constituent of vitrification solutions. Due to their dense branching, Ficolls also serve as ideal, nearly spherical molecular crowding agents.
FicollTM PM 70 is used as a macromolecular crowding agent in studies of cell volume signaling and protein refolding.[1]It may be used in tissue engineering and macromolecular conformation research for the development, evaluation, and use of macromolecular crowding (MMC) systems and configurations. Ficoll PM-70 can also be used in vitrification research.

Other Notes

A nonionic synthetic polymer of sucrose.
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Legal Information

Ficoll is a registered trademark of Cytiva

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Este artículo
GE17-0310-10GE17-0300-10GE17-0310-50
Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

solubility

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

solubility

soluble

solubility

soluble

solubility

soluble

form

powder

form

solid, spray-dried

form

solid, spray-dried

form

solid, spray-dried

color

white

color

White

color

White

color

White

mol wt

~70,000

mol wt

60-80 kDa

mol wt

-

mol wt

60-80 kDa

type

Type 70

type

-

type

-

type

-


Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Protocolos

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.


M B Burg
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 10(5-6), 251-256 (2000-12-23)
The non-ideal properties of solutions containing high concentrations of macromolecules can result in enormous increases in the activity of the individual macromolecules. There is considerable evidence that macromolecular crowding and confinement not only occur in cells, but that these are
M Al-Habori
The international journal of biochemistry & cell biology, 33(9), 844-864 (2001-07-20)
Macromolecular crowding has been proposed as a mechanism by means of which a cell can sense relatively small changes in volume or, more accurately, the concentration of intracellular solutes. According to the macromolecular theory, the kinetics and equilibria of enzymes
Y Georgalis et al.
Journal of colloid and interface science, 386(1), 141-147 (2012-08-23)
Ficoll PM70 is an important branched polysaccharide polymer with a variety of applications in biology and biophysics. The accepted picture for the aqueous solutions of this polymer is that of a polydisperse nearly spherical suspension with a molecular weight of



Número de artículo de comercio global

SKUGTIN
F2878-100G04061833613344
F2878-50G04061842397082
F2878-250G04061842397075
F2878-500G04061833613368

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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