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GE17-0300-10

Ficoll® PM 400

Cytiva 17-0300-10, pack of 100 g

Sinónimos:

Density gradient medium

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About This Item

UNSPSC Code:
12352201
NACRES:
NA.25

form

solid
spray-dried

packaging

pack of 100 g

manufacturer/tradename

Cytiva 17-0300-10

color

White

solubility

soluble

General description

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media.
It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained

Features and Benefits

  • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
  • Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
  • Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
  • Better osmotic properties than sucrose.
  • Preserves functional and morphological integrity.
  • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
  • Does not penetrate biological membranes.
  • Serves as the raw material for preparation of Ficoll-Paque gradients.
  • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class

13 - Non Combustible Solids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

How to Fractionate and Analyze Gradients of Percoll

Density Marker Beads facilitate Percoll gradient monitoring in biological separations.

Protocolos

Recommended Standard Method for Isolating Mononuclear Cells

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

How to Make and Use Percoll Gradients

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

Contenido relacionado

Applications of Percoll In Blood Cells

Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico