D8899

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

• Sinónimos: DNA single stranded
• Número CAS: 91080-16-9
• UNSPSC Code: 41106310
• eCl@ss: 32160414
• EC Number: 293-507-3
• NACRES: NA.52
• MDL number: MFCD00163436
• Biological source: bovine (calf) thymus
• Form: lyophilized powder
• Storage temp.: −20°C

Propiedades

• biological source: bovine (calf) thymus
• Quality Segment: 200
• grade: Molecular Biology
• form: lyophilized powder
• mol wt: ~50 kb
• composition: ssDNA, ≥65%
• storage temp.: −20°C

Descripción

General description

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay[1] and in DNA-binding assay.[2]

Features and Benefits

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Preparation Note

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

One mg DNA is approx. 25 A₂₆₀ units.

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves