The molecular weight for D4522 is reported to be between 10-15 million Daltons prior to activation (nicking). 15,000 kilodaltons x (1kb/660 kilodaltons) = 22.72 kb (on average). The average number of base pairs is not measured, and therefore, this calculation is an estimate. We do not have a molecular weight measurement after it's been nicked, but it is expected to be a range below 22.72 kB. We do not have comparison data to inform us of how they may respond differently as a standard curve for a fluorescent assay. We would suggest checking to see if the mechanics behind your assay are length dependent or could be influenced by the ends being nicked or not. Products like D4764 that are deemed stable for long periods of time do not have a noted expiration or retest date.
D4522
Deoxyribonucleic acid from calf thymus
Type XV, Activated, lyophilized powder
Sinónimos:
Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|
Acerca de este artículo
biological source
bovine pancreas
Quality Segment
grade
Molecular Biology
type
Type XV
form
lyophilized powder
solubility
water: slightly soluble
storage temp.
−20°C
General description
Application
Features and Benefits
• High quality template DNA
Preparation Note
1 of 1
Este artículo | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade - |
| biological source bovine pancreas | biological source bovine (calf) thymus | biological source bovine (calf) thymus | biological source bovine (calf) thymus |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| solubility water: slightly soluble | solubility - | solubility - | solubility - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
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Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| D4522-1MG | 04061833565384 |
| D4522-5MG | 04061833565391 |
| D4522-5X5MG | 04061833565407 |
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Could you please confirm whether this product is suitable to be used as a DNA standard for generating a calibration curve (0–200 ng/µL) in such applications? DNA quantification using the Hoechst 33258 dye in a fluorescence-based assay.
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Could you provide the number of base pairs for the D4522 product? For example, is it around 20kb?
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The reported molecular weight is between 10-15 million Daltons before activation (nicking), primarily as double-stranded DNA. A double-stranded DNA of 1 kb in length has a molecular weight of 660 kiloDalton. A size of 15 million daltons is equivalent to 15,000 kilodaltons. When multiplied by (1kb/660 kilodaltons), it equals 22.72 kb on average. It's important to note that the average number of base pairs is not measured, so this calculation provides an estimate.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Product D4522, Deoxyribonucleic acid?
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The molecular weight is reported to be between 10-15 million Daltons prior to activation (nicking).
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What is the solution stability of Product D4522, Deoxyribonucleic acid?
1 answer-
Solutions of deoxyribonucleic acid have been stored successfully for several months at 4°C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (25 μg/mL), DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store deoxyribonucleic acid in alkaline solutions since it tends to degrade at pH greater than 8.0.
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Is BSA (bovine serum albumin) used during the method of preparation for Product D4522, Deoxyribonucleic acid?
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During the method of preparation, when deoxyribonucleic acid is nicked, BSA is added to the solution. This is followed by an extraction method to remove all exogenous protein. We do not, however, claim this deoxyribonucleic acid is 100% clean of all proteins (including BSA).
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Is Product D4522, Deoxyribonucleic acid, single or double stranded?
1 answer-
Even though it is activated or "nicked" DNA, we have found that it is primarily double stranded.
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