Saltar al contenido
Merck

Documentos clave

Ver Toda la documentación

Saltar a

D2532

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

Sinónimos:

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas
Tamaño de envaseSKUDisponibilidadPrecio
5 mL
Comprobar disponibilidad del carrito
$143.000
5 x 5 mL
Comprobar disponibilidad del carrito
$483.000

Acerca de este artículo

NACRES:
NA.25
UNSPSC Code:
12161700

$143.000

Comprobar disponibilidad del carrito
​
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarle

Quality Segment

300

form

solution

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Preparation Note

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 1

Este artículo
D9905S4030H7033
Denhardt’s Solution 50x for Northern and Southern blotting, solution

Sigma-Aldrich

D2532

Denhardt′s Solution 50x

Denhardt’s Solution for Northern and Southern blotting, lyophilized powder

Sigma-Aldrich

D9905

Denhardt′s Solution

Dextran Sulfate 50% Solution

Millipore

S4030

Dextran Sulfate 50% Solution

PerfectHyb™ Plus Hybridization Buffer for Northern and Southern blotting, solution

Sigma-Aldrich

H7033

PerfectHyb™ Plus Hybridization Buffer

technique(s)

Southern blotting: suitable

technique(s)

Southern blotting: suitable

technique(s)

hybridization: suitable (nucleic acid)

technique(s)

-

Quality Level

300

Quality Level

100

Quality Level

100

Quality Level

200

form

solution

form

lyophilized powder

form

-

form

solution

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

storage temp.

room temp

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

-

foreign activity

-

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000484704
0000484704
0000411631
0000411631
0000375543

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Protocolos

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Contenido relacionado

Número de artículo de comercio global

SKUGTIN
D2532-5X5ML04061838255143
D2532-5ML04061838104885

Questions

  1. Can you share the CAS # for the components (Ficoll and PVP) that are used to make the 50X Denhart's Solution?

    1 answer

    1. The CAS number for the Ficoll ingredient is 26873-85-8. The CAS number for the PVP ingredient is 9003-39-8 2.

      Below is the link to the Product Data Sheet for more information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/172/670/d2532dat.pdf

      Helpful?

Reviews

No rating value

Active Filters