Conn's Biological Stains, 10th Edition states excitation is at 494 nm in aqueous solutions at pH 9; the emission maximum is 517 nm. Depending upon the pH of the solution, slightly different values have been reported in the literature.
C0875
Calcein
Powder
Sinónimos:
Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon
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$324.000
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$595.000
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$1.280.000
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5 G
$324.000
10 G
$595.000
25 G
$1.280.000
About This Item
Fórmula empírica (notación de Hill):
C30H26N2O13
Número de CAS:
Peso molecular:
622.53
Beilstein:
8181068
Número MDL:
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
NACRES:
NA.47
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Nombre del producto
Calcein, Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.
Nivel de calidad
descripción
mixed isomers
Formulario
powder
técnicas
titration: suitable
color
yellow to brown
solubilidad
NaOH: 50 mg/mL
fluorescencia
λex 470 nm; λem 509 nm (pH 7.4)
idoneidad
suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg)
aplicaciones
diagnostic assay manufacturing
hematology
histology
temp. de almacenamiento
room temp
cadena SMILES
OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O
Clave InChI
DEGAKNSWVGKMLS-UHFFFAOYSA-N
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Aplicación
Acciones bioquímicas o fisiológicas
Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions.[4] Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.[5]
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
Lot/Batch Number
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Artículos
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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What are the excitation and emission wavelengths of Calcein, Product C0875?
1 answer-
Helpful?
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Is there a reference for using Calcein, Product C0875, to study bone growth?
1 answer-
Clement-Lacroix, P., et al. Osteoblasts are a new target for prolactin: analysis of bone formation in prolactin receptor knockout mice. Endocrinology, 140, 96-105 (1999).
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the solubility of Calcein, Product C0875?
1 answer-
Calcein is soluble at a concentration of 50 mg/mL in 1M sodium hydroxide, yielding clear orange to brown solution.
Helpful?
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What is the extinction coefficient of Calcein, Product C0875?
1 answer-
For samples in 1 M sodium hydroxide: E1% = 1040 at 500-504 nm; E1% = 466 at 242-245 nm.
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