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Merck

M1255

Sigma-Aldrich

Calcein Blue

Powder

Sinónimos:

4-Methylumbelliferone-8-methyliminodiacetic acid

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1 G
$126.000
10 G
$663.000

$126.000


Fecha estimada de envío30 de abril de 2025


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1 G
$126.000
10 G
$663.000

About This Item

Fórmula empírica (notación de Hill):
C15H15NO7
Número de CAS:
Peso molecular:
321.28
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
NACRES:
NA.47

$126.000


Fecha estimada de envío30 de abril de 2025


Solicitar un pedido a granel

Nombre del producto

Calcein Blue, fluorescent dye

Nivel de calidad

Formulario

powder

técnicas

titration: suitable

color

white to light yellow

solubilidad

1 M NaOH: 50 mg/mL

fluorescencia

λex 322 nm; λem 445 nm in methanol

idoneidad

suitable for indicator for EDTA titration

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12

InChI

1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)

Clave InChI

RJPSHDMGSVVHFA-UHFFFAOYSA-N

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Descripción general

Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.

Aplicación

Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves


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Questions

  1. Is Calcein Blue identical to Calcein Blue AM, and if not, what is the difference? Would it be compatible in a flourescent microscopy assay looking at %viability of THP-1 cells?

    1 answer
    1. product M1255, Calcein Blue to is used to stain cells, but it will stain living or dead cells. For a cell viability stain, one can use Calcein Blue AM. The difference between Calcein Blue and Calcein Blue AM is the methyl-iminodiaceitc acid group that chelates the divalent metal has acetoxymethyl esters on it in Calcein Blue AM. The esters are cleaved inside of viable cells, but remain on the molecule in the absence of active esterases. So, if you use M1255 it will stain all the cell whether alive or dead.

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