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A8275

IgG anti-conejo (molécula completa)-Peroxidasa antibody produced in goat

Name: IgG anti-conejo (molécula completa)-Peroxidasa antibody produced in goat
Brand: SIGMA
Price: 200000 CLP

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:

Goat Anti-Rabbit IgG (whole molecule)–HRP

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Tamaño de envase	SKU	Disponibilidad	Precio
1 mL		Comprobar disponibilidad del carrito	$200.000

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UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162788

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d)

Citations:

$200.000

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Propiedades

biological source

goat

Quality Level

200

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Descripción

General description

IgG, a monomer, is an antibody circulating in blood and can assist in phagocytic destruction of foreign microorganism. It plays an important role in Antibody dependent cell mediated cytotoxicity and is also associated with type II and type III hypersensitivity. Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody can be used in pAb-ELISA. Rabbit anti-IgG antibody coupled with peroxidase (1:2500 dilution in TBS, 0.5% BSA) can be used in western blots. This antibody is also used as detector in indirect ELISA for saxitoxin analysis. Goat anti-rabbit IgG Peroxidase conjugate reacted specifically with all rabbit immunoglobulin.

Immunogen

Purified rabbit IgG.

Application

Se realizaron análisis de inmunoprecipitación y inmunoelectrotransferencia (técnica western) de los lisados celulares C33A utilizando IgG anti-conejo de cabra conjugada con HRP como el anticuerpo secundario.

Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody is used in western blotting, Indirect ELISA, Immunoblotting and Immunodetection.

Immunohistochemistry was performed on frozen sections (10um) of mouse intestine, liver, and spleen using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Prior to incubation with the secondary, sections were treated with a mixture of MeOH/hydrogen peroxide 30% to block endogenouse peroxidases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Este artículo	A0545	A8667	A3812
$Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A8275 Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat	Sigma-Aldrich A0545 Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat	$Anti-Human IgG (whole molecule)−Peroxidase antibody produced in goat IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A8667 Anti-Human IgG (whole molecule)−Peroxidase antibody produced in goat	Sigma-Aldrich A3812 Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat
technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)	technique(s) direct ELISA: 1:30,000, dot blot: 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate alkaline phosphatase conjugate
antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
biological source goat	biological source goat	biological source goat	biological source goat
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in wet ice
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C

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pictograms

GHS07,GHS09

signalword

Warning

hcodes

H315,H317,H319,H411

pcodes

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

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Número de artículo de comercio global

SKU	GTIN
A8275-.5ML	04061835805716
A8275-1ML	04061833391389
A8275-2ML	04061835805723

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