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Merck

45359

Sigma-Aldrich

Epoxy Embedding Medium kit

embedding resin for electron microscopy

Sinónimos:

Epon substitute embedding medium kit

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

pH

5-7

density

1.9 g/cm3

application(s)

hematology
histology

Storage temp.

2-8°C

General description

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Application

Epoxy Embedding Medium Kit has been used in the following applications:

  • to study the role of NDRG2, an essential colonic epithelial barrier regulator, in gut homeostasis maintenance and colitis-associated tumor development
  • to study the interaction of polymer scaffold nanofibers with growing cells
  • to study the morphological characterization of postembryonic development of blood–spleen barrier in ducks
  • to understand the role of NOD1/NOD2 receptors in Fusobacterium nucleatum-mediated neutrophil extracellular traps

Features and Benefits

  • Excellent medium for embedding a wide variety of tissues, including both plant and animal.
  • Facilitates rapid embedding (less than 3 hours).
  • Low viscosity facilitates rapid tissue penetration.
  • Desirable hardness is easy to obtain to suit a specific tissue type.

Components

45345 Epoxy-Einbettungsmittel 2x250 ml
45347 Härter MNA 250 ml
45356 Härter DDSA 250 ml
45348 Beschleuniger DMP 30 250 ml

Legal Information

Epon is a trademark of Hexion, Inc.

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Referencia del producto
Descripción
Precios

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

275.0 °F

flash_point_c

135 °C


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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A Sawaguchi et al.
Journal of microscopy, 234(2), 113-117 (2009-04-29)
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High resolution 3D microscopy study of cardiomyocytes on polymer scaffold nanofibers reveals formation of unusual sheathed structure
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Bacteria are generally difficult specimens to prepare for conventional resin section electron microscopy and mycobacteria, with their thick and complex cell envelope layers being especially prone to artefacts. Here we made a systematic comparison of different methods for preparing Mycobacterium

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