X4875

Xanthine Oxidase from bovine milk

Grade IV, ammonium sulfate suspension, ≥0.1 units/mg protein

• Sinónimos: XOD, Xanthine:oxygen oxidoreductase
• Número de CAS: 9002-17-9
• Comisión internacional de enzimas: 1.17.3.2.
• EC Number: 232-657-6
• MDL number: MFCD00082145
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• biological source: bovine milk
• type: Grade IV
• form: ammonium sulfate suspension
• specific activity: ≥0.1 units/mg protein
• storage temp.: 2-8°C

Descripción

General description

Research area: Cell CycleXanthineoxidase (XO) is a homodimer. It is made up of two sub-units each made up of onemolybdenum and two iron-sulphur (2Fe-2S) centers.

enthält auch proteolytische Aktivität

Application

Xanthine Oxidase from bovine milk has been used in superoxide dismutaseactivity assay.Xanthine oxidase may be suitable for producing amperometric biosensors and designing-flavonoid-type inhibitors against XO for treating inflammatory diseases, gout, and hyperuricemia. It may also be used in the synthesis of superoxide because of aldehyde oxidation.

Biochem/physiol Actions

Although xanthine oxidase is found widely distributed in multiple tissues, it is highly expressed in endothelial and epithelial cells. Its expression was also increased in infected tissues where it was shown to have an anti-microbial effect due to its ability to generate reactive oxygen species. Xanthineoxidase plays an important role in catalyzing the hydroxylation ofhypoxanthine to Xanthine. Later, xanthine is converted to uric acid isexcreted by the kidneys.

Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Suspension in 2.3 M (NH₄)₂SO₄ containing 1 mM sodium salicylate

Analysis Note

Protein determined by biuret

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)