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SHC002

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA

Targets no known mammalian genes

Sinónimos:

MISSION® Control Vectors

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UNSPSC Code:
41106609
NACRES:
NA.51
MDL number:
Concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
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Quality Segment

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

Application

MISSION® pLKO.1-puro non-mammalian shRNA control plasmid DNA has been used:
  • to transduce RLE-6TN cells[1]
  • for the validation of Oct4-green fluorescent protein (GFP) mouse embryonic fibroblasts (MEFs)
  • as a negative control for RNA knockdown

MISSION®pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA has been used:
  • for the knockdown of transmembrane prostate androgen-induced RNA (TMEPAI)
  • in the infection mixture for lentivirus preparation[1]
  • for generating the puromycin-resistant ST14A (rat striatal neuronal cell line) cell populations
  • to induce Ago2 (argonaute-2) down-regulation

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este artículo
SHC202SHC005SHC007
product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Número de artículo de comercio global

SKUGTIN
SHC00204061826600283

Questions

1–2 of 2 Questions  
  1. what is the TRCN ID for MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA (SHC002)

    1 answer
    1. This product does not have a TRCN ID associated with it. The non-targeting sequence expressed in the hairpin is CAACAAGATGAAGAGCACCAA.

      Helpful?

  2. What is the antibiotic resistance for bacterial transformations in SHC002?

    1 answer
    1. The antibiotic resistance for bacterial transformations in SHC002 includes an ampicillin resistance cassette. However, it is recommended to use the more stable analog, carbenicillin.

      Helpful?

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