P6911

Proteasa from Streptomyces griseus

BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

• Sinónimos: Actinasa E, Pronasa E
• Número CAS: 9036-06-0
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-909-5
• NACRES: NA.21
• MDL number: MFCD00132092
• Concentration: ≥4 unit/mg

Propiedades

• grade: Molecular Biology
• Quality Segment: 200
• product line: BioReagent
• form: powder
• mol wt: monomer ~20 kDa
• concentration: ≥4 unit/mg
• solubility: water: 5-20 mg/mL
• foreign activity: DNase, RNase, and nickase, none detected (No RNase.)
• storage temp.: −20°C

Descripción

General description

Completamente inactivada al calentarse por encima de 80 °C durante 15 a 20 minutos.

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

Application

Suitable for:

• Nucleic acid isolation procedures in incubations
• Degrade protein during nucleic acid purification
• Proteolysis of insoluble protein
• Structural protein studies

Esta enzima es más activa a un intervalo de pH más elevado que la proteasa alcalina conocida, mostrando actividad proteolítica incluso en disolución de NaOH 0,2 N. Esta enzima es útil para la proteólisis de proteínas insolubles y para la investigación de la estructura de las proteínas.

La proteasa se utiliza normalmente en los procedimientos de aislamiento de ácidos nucleicos en incubaciones de 0,5 a 3,0 horas complementadas con dodecilsulfatosódico al 0,2 % y EDTA 10mM.

Biochem/physiol Actions

Mezcla de como mínimo tres actividades proteolíticas, entre ellas una serín proteasa extracelular. En general, las proteasas de serina exhiben una amplia variedad de especificidades de sustrato, que se creen mediadas por un centro activo compuesto de un resto de Asp, uno de His y uno de Ser en la molécula. Esta enzima prefiere hidrolizar los enlaces peptídicos del lado carboxilo de los ácidos glutámico o aspártico.

Preparation Note

Recolectada del caldo de cultivo de S. griseus.

Analysis Note

The protease is incubated for 10 minutes at pH 7.5 at 37°C in a 6 ml reaction volume containing 0.54% casein and 0.041 M potassium phosphate buffer. The reaction is stopped by the addition of 5.0 ml of 0.11 M trichloroacetic acid.

Other Notes

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

This protease is completely inactivated by heating above 80°C for 15-20 minutes. This enzyme is more active at a higher pH range, showing the proteolytic activity even in 0.2N NaOH solution.

Información de seguridad

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)