P5306

Papaína from papaya latex

lyophilized powder, aseptically filled

• Sinónimos: Papinasa
• Número CAS: 9001-73-4
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-627-2
• MDL number: MFCD00131791
• Número CE: 3.4.22.2 (BRENDA, IUBMB)
• eCl@ss: 42030311
• Specific activity: ≥8.0 units/mg protein
• Biological source: papaya (latex)

Propiedades

• biological source: papaya (latex)
• Quality Segment: 200
• sterility: aseptically filled
• form: lyophilized powder
• specific activity: ≥8.0 units/mg protein
• mol wt: 23.4 kDa
• solubility: H₂O: soluble 1.2 mg/mL
• storage temp.: −20°C

Descripción

General description

Papain is a thermostable enzyme and becomes inactive at 55°C. It has two domains and the catalytic region is present at the domain interface. The cysteine 25 in the active site performs the proteolysis by nucleophilic attack on the carbonyl carbon of a peptides. Papain has wide industrial applications in food and pharmaceutical industry.

Application

Utilizada para producir fragmentos Fab de los anticuerpos. Se utiliza también para la disociación celular, ya que se ha demostrado que es más eficaz y menos lesiva con ciertos tejidos.

The product from Sigma has been used in the digestion of extracellular matrix (ECM).[1] It has also been used with other proteolytic enzymes for in situ protein degradation to produce crystals suitable for structure determination.[2]

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies . It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P5306 is provided as a lyophilized powder that is aseptically filled. It has been used to digest mouse retinas to study the RAGE axis in nonproliferative diabetic retinopathy (NPDR)[1].

Biochem/physiol Actions

Una proteasa de cisteína que rompe los enlaces peptídicos de los aminoácidos básicos, la leucina o la glicina
pH óptimo 6,0-7,0
También hidroliza ésteres y amidas.

Papain papaya latex has antifungal activity against C. albicans. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

Preparation Note

Prepared from P 4762

Other Notes

Véase más información sobre la papaína en www.sigma-aldrich.com/enzymeexplorer.

One unit will hydrolyze 1.0 μmole of BAEE per minute at pH 6.2 at 25 deg C.

Información de seguridad

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves