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M8042

Medio esencial mínimo Eagle

Name: Medio esencial mínimo Eagle
Brand: SIGMA

Alpha Modification, with nucleosides and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

MEM

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UNSPSC Code:

12352207

NACRES:

NA.75

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Propiedades

Nombre del producto

Medio esencial mínimo Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

Hanks’ salts (2% CO₂): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO₂): yes
NaHCO₃: yes

shipped in

ambient

storage temp.

2-8°C

Descripción

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Often used in cytogenetic analysis when supplemented with fetal bovine serum (FBS).

Application

Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell, and osteoblast.

Minimum Essential Medium Eagle is used for primary osteoblast cultures[1] and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.[2]

Biochem/physiol Actions

MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO₃, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.[3]

Preparation Note

Supplement with 0.292 g/L L-glutamine

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Este artículo	M0450	M0200	M2279
Sigma-Aldrich M8042 Medio esencial mínimo Eagle	Sigma-Aldrich M0450 Medio esencial mínimo Eagle	Sigma-Aldrich M0200 Medio esencial mínimo Eagle	Sigma-Aldrich M2279 Medio esencial mínimo Eagle
form liquid	form liquid	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
components Hanks’ salts (2% CO₂): no glucose: yes sodium pyruvate: yes phenol red: yes HEPES: no L-glutamine: no stable glutamine: no Earle’s salts (5% CO₂): yes NaHCO₃: yes	components glucose: 1.0 g/L L-glutamine: 0.292 g/L phenol red: 0.011 g/L NaHCO₃: 2.2 g/L	components phenol red: 0.011 g/L glucose: 1.0 g/L L-glutamine: 0.292 g/L NaHCO₃: 2.2 g/L	components phenol red: yes sodium pyruvate: no L-glutamine: no HEPES: no NaHCO₃: yes Earle’s salts (5% CO₂): yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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