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M4655

Medio esencial mínimo Eagle

Name: Medio esencial mínimo Eagle
Brand: SIGMA

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Minimum Essential Medium Eagle

Sinónimos:

MEM

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NACRES:

NA.75

UNSPSC Code:

12352207

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Propiedades

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO₂): yes
HEPES: no
NaHCO₃: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Descripción

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser.

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Este artículo	M2414	FG0325	M0325
Sigma-Aldrich M4655 Minimum Essential Medium Eagle	Sigma-Aldrich M2414 Minimum Essential Medium Eagle	SAFC FG0325 Minimum Essential Medium Eagle	Sigma-Aldrich M0325 Minimum Essential Medium Eagle
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
form liquid	form liquid	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components sodium pyruvate: no phenol red: yes Earle’s salts (5% CO₂): yes HEPES: no NaHCO₃: yes L-glutamine: yes	components phenol red: 0.011 g/L NaHCO₃: 0.85 g/L glucose: 1.0 g/L L-glutamine: no	components stable glutamine: yes L-glutamine: no phenol red: yes sodium pyruvate: no Earle’s salts (5% CO₂): yes HEPES: no Hanks’ salts (2% CO₂): no glucose: yes NaHCO₃: yes	components glucose: 1.0 g/L (Dextro) L-glutamine: 0.292 g/L phenol red: 0.011 g/L Earle’s salts (5% CO₂): yes NaHCO₃: 2.2 g/L
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Artículos

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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Número de artículo de comercio global

SKU	GTIN
M4655-500ML	04061837847387
M4655-6X1L	04061834059134
M4655-1L	04061834059110
M4655-6X500ML	04061837847394
M4655-10L	04061837340796
M4655-24X500ML	04061834059127