If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
L4516
Lipopolysaccharides from Escherichia coli O127:B8
BioXtra, suitable for cell culture, γ-irradiated
Sinónimos:
LPS
Seleccione un Tamaño
| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 1 mg | Comprobar disponibilidad del carrito | $65.500 |
Acerca de este artículo
biological source
Escherichia coli (O127:B8)
Quality Segment
sterility
γ-irradiated
product line
BioXtra
form
lyophilized powder
purified by
gel-filtration chromatography
technique(s)
cell culture | mammalian: suitable
impurities
<1% Protein (Lowry)
solubility
H2O: 5 mg/mL, slightly hazy
storage temp.
2-8°C
Application
- to study the LPS-induced macrophage survival in mice[1]
- to elicit the secretion of cytokines by cattle PBMC[2]
- to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environment[1]
- to perform autophagy assay in BV2 and BV2-LC3 cells[3]
- for stimulation of human monocytes, macrophages and mouse microglia cells[4][5][6][7]
- to induce pentraxin-3 expression in 293T cells[8]
Biochem/physiol Actions
Preparation Note
Other Notes
1 of 1
Este artículo | |||
|---|---|---|---|
| biological source Escherichia coli (O127:B8) | biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O111:B4) | biological source Escherichia coli (O55:B5) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell based assay: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| solubility H2O: 5 mg/mL, slightly hazy | solubility H2O: 5 mg/mL, slightly hazy | solubility H2O: 5 mg/mL, slightly hazy | solubility H2O: 5 mg/mL, faintly hazy to hazy |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Still not finding the right product?
Explore all of our products under Lipopolysaccharides from Escherichia coli O127:B8
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral
Clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Artículos
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
Contenido relacionado
Datasheet
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| L4516-1MG | 04061833957578 |
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
Once reconstituted what is the stability at -20C for the LPS product L4516-1M?
1 answer-
Frozen aliquots can be stored up to 2 years. For more information, please see the link below:
https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharidesHelpful?
-
-
Can LPS bind to plastics (ex. 1.5 mL microtube) even when LPS solution is frozen stored at -20℃? I store LPS solution at 0.05mg/mL in -20℃ now.
1 answer-
LSP is less likely to stick to plastics than glass, however very low concentrations (<0.1 mg/mL in water), LPS tends to stick to containers made
of certain types of glass or plastic. To minimize, prepare stock solutions of at least 1 mg/mL. If the LPS concentration is >1 mg/mL, adsorption to the sides of the vial is
negligible. For solutions stored in glass, vortexing for 30 minutes following thaw is recommended to redissolve. This may be useful for dilute solutions in plastic, as well. Surfaces (glass and plastics) may be salinized to reduce adherence.See Sigmacote, SL2:
https://www.sigmaaldrich.com/product/sigma/sl2For additional information, see the link below to review the Lipopolysaccharides product information page:
https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharidesHelpful?
-