As listed in the 'PROPERTIES' section, the composition in g/L is the following:
Agar, 15 g/L
NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
L3147
LB Broth with agar (Miller)
Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.
Sinónimos:
Luria Agar, Luria Bertani agar, Miller’s LB agar, lysogeny broth agar
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 250 g | Comprobar disponibilidad del carrito | $119.000 | |
| 1 kg | Disponible para enviar HOYdesdePUDAHUEL | $562.000 |
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UNSPSC Code:
41106200
NACRES:
NA.85
eCl@ss:
42040102
$119.000
Comprobar disponibilidad del carrito
Servicio técnico
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Molecular Biology
Quality Segment
sterility
non-sterile
form
powder
composition
Agar, 15 g/L , NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(4% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.
Application
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
LB Broth with agar (Miller) has been used for the generation of single colony for Pseudomonas putida KT2440, plating E. coli and for the generation of pure colonies of C14 and MC8M6 of bacterial strains.
Features and Benefits
Miller′s LB powder with agar provides:
- Easy scale-up using larger package sizes
- A budget-friendly alternative to pre-poured plates
- Standard formulation
Preparation Note
1. Suspend 40 g in 1L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into sterile petri dishes.
To prepare the medium of Luria and Burrows: Add 1 g glucose to medium and proceed with preparation instructions above.
To prepare the medium of Luria, Adams and Ting (also known as LC agar): Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into sterile petri dishes.
To prepare the medium of Luria and Burrows: Add 1 g glucose to medium and proceed with preparation instructions above.
To prepare the medium of Luria, Adams and Ting (also known as LC agar): Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.
Add 40g powder to 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired).
1 of 1
Este artículo | |||
|---|---|---|---|
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| form powder | form powder | form powder | form liquid |
| technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| composition Agar, 15 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L , NaCl, 10 g/L | composition NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition - |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility sterile; 0.2 μm filtered |
| application(s) food and beverages | application(s) food and beverages | application(s) food and beverages | application(s) microbiology |
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocolos
General protocols for growth of competent cells in microbial medium.
Artículos
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| L3147-1KG | 04061835573455 |
| L3147-250G | 04061833950883 |
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How many grams per liter?? No info on bottle.
1 answer-
Helpful?
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Can you provide the expiry date for my media? I couldn't find it on the Certificate of Analysis (COA) or the bottle.
1 answer-
The expiry date information can be found on the Certificate of Analysis (COA) or the product packaging. If there is no expiry date information available, your product is covered by a one-year warranty starting from the date it was received at your site.
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There is no instruction on the bottle for LB Broth with agar (L3147-1kg). how much powder medium I should use for making 1L agar?
1 answer-
Preparation instructions for this product are published on the product page under Reconstitution. To reconstitute, add 40g powder to 1 L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired).
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the difference among the LB - Miller products?
1 answer-
LB - Miller is available in many types to suit your needs. The different product formats include powder and liquid form. The powder form is also available with agar for easy LB-agar plate preparation.L2542 (LB Miller liquid)L3522 (LB Miller powder)L3147 (LB Miller powder with agar)
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What are the differences among the Luria, Lennox and Miller LB formulations?
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LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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