No, this product is not tested for use on the CFX96 Biorad system, but there is a different KiCqStart mix - KCQS07, which is compatible with the CFX96 instrument.
KCQS08
KiCqStart® One-Step Probe RT-qPCR ReadyMix™, Low ROX™
with Low ROX for ABI and Stratagene instruments
Sinónimos:
KiCqStart® One Step Probe RT-qPCR Readymix™
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 500 reactions | Comprobar disponibilidad del carrito | $405.000 |
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NACRES:
NA.55
UNSPSC Code:
41106300
Servicio técnico
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liquid
usage
sufficient for 500 reactions
feature
dNTPs included, hotstart
storage condition
protect from light
technique(s)
RT-qPCR: suitable, qPCR: suitable
color
light blue
input
purified RNA
compatibility
ABI 7500 Fast, ABI 7500, ABI QuantStudio, ABI ViiA 7, Agilent AriaMx, Douglas Scientific IntelliQube, Qiagen Corbett Rotor-Gene Q, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000
detection method
probe-based
shipped in
dry ice
storage temp.
−20°C
General description
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5′-hydrolysis probes on real-time PCR systems (refer to instrument compatibility section to select the appropriate reagent for your machine). First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times.
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries. The reverse transcriptase is an engineered M-MLV with reduced RNase H activity and improved activity and stability at higher temperatures. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is highly resistant to PCR inhibitors. A key component of the ReadyMix™ is an ultra-pure, highly processive, thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This provides an extremely stringent automatic hot-start that minimizes the potential for primer-dimer and other non-specific PCR artifacts. The light blue color of the inert dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. The dye does not interfere with qPCR performance or affect the stability of the product.
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries. The reverse transcriptase is an engineered M-MLV with reduced RNase H activity and improved activity and stability at higher temperatures. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is highly resistant to PCR inhibitors. A key component of the ReadyMix™ is an ultra-pure, highly processive, thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This provides an extremely stringent automatic hot-start that minimizes the potential for primer-dimer and other non-specific PCR artifacts. The light blue color of the inert dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. The dye does not interfere with qPCR performance or affect the stability of the product.
Application
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart® One-Step Probe RT-qPCR ReadyMix™ does not contain an internal reference dye. Compatible instruments include:
- Applied Biosystems® 7500
- Applied Biosystems 7500 Fast
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio
- Agilent® AriaMx
- Douglas Scientific IntelliQube
- QIAGEN® Rotor-Gene® Q
- Stratagene Mx3000P®
- Stratagene Mx3005P®
- Stratagene Mx4000™
Features and Benefits
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
Other Notes
2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers
packaging:
500 reactions* = 5 X 1 mL tubes
*number of reactions based on a 20uL volume
packaging:
500 reactions* = 5 X 1 mL tubes
*number of reactions based on a 20uL volume
Legal Information
Agilent is a registered trademark of Agilent Technologies, Inc.
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
Mx3000P is a registered trademark of Agilent Technologies, Inc.
Mx3005P is a registered trademark of Agilent Technologies, Inc.
Mx4000 is a trademark of Agilent Technologies, Inc.
Qiagen is a registered trademark of Qiagen Group
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
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Este artículo | |||
|---|---|---|---|
| technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable |
| color light blue | color light blue | color light blue | color light blue |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition protect from light |
| compatibility ABI 7500 Fast, ABI QuantStudio, Agilent AriaMx, Qiagen Corbett Rotor-Gene Q, Strategene Mx3005P, Douglas Scientific IntelliQube, Strategene Mx4000, ABI ViiA 7, Strategene Mx3000P | compatibility Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MiniOpticon, Eppendorf® Mastercycler ep realplex2 s, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480 | compatibility ABI 5700, ABI 7000, ABI 7300, ABI 7700, ABI 7900 HT , ABI 7900 HT Fast, ABI StepOne, ABI StepOnePlus | compatibility ABI 7500 Fast, ABI QuantStudio, ABI ViiA 7 , Agilent AriaMx, Douglas Scientific IntelliQube, Qiagen Corbett Rotor-Gene Q, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000 |
| form liquid | form liquid | form liquid | form liquid |
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocolos
KiCqStart One-Step Probe RT-qPCR ReadyMix streamlines RNA template RT-qPCR with high sensitivity.
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Instructions
R Grace Walton et al.
Physiological reports, 3(6), doi:10-doi:10 (2015-06-04)
Reduced vessel density in adipose tissue and skeletal muscle is associated with obesity and may result in decreased perfusion, decreased oxygen consumption, and insulin resistance. In the presence of VEGFA, Angiopoietin-2 (Angpt2) and Angiopoietin-1 (Angpt1) are central determinants of angiogenesis
Tyler J Kirby et al.
Physiological genomics, 48(2), 145-153 (2015-12-17)
Adipose tissue has profound effects on whole-body insulin sensitivity. However, the underlying biological processes are quite complex and likely multifactorial. For instance, the adipose transcriptome is posttranscriptionally modulated by microRNAs, but the relationship between microRNAs and insulin sensitivity in humans
Tyler J Kirby et al.
Molecular biology of the cell, 27(5), 788-798 (2016-01-15)
Myofibers increase size and DNA content in response to a hypertrophic stimulus, thus providing a physiological model with which to study how these factors affect global transcription. Using 5-ethynyl uridine (EU) to metabolically label nascent RNA, we measured a sevenfold
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| KCQS08-SAMPLE | 04061838113924 |
| KCQS08-500RXN | 04061833945209 |
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Does this product work on the cfx96 biorad?
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