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I3146

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Sinónimos:

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

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Tamaño de envaseSKUDisponibilidadPrecio
5 mL
Comprobar disponibilidad del carrito
$52.200

Acerca de este artículo

NACRES:
NA.75
UNSPSC Code:
12352205

$52.200


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sterility

sterile-filtered

Quality Segment

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

Application

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
  • α mouse liver 12 (AML-12) cells[1]
  • rat fetal testis explants[2]
  • human dental pulp stem cells (DPSC)[3]

Biochem/physiol Actions

  • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.[4]
  • Transferrin: an iron-transport protein.
  • Selenium: an essential trace element normally provided by serum.[5]

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Other Notes

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

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Este artículo
I2521I2771I0516
technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

form

liquid

form

liquid

form

liquid

form

solution

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested


Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Artículos

Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

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Número de artículo de comercio global

SKUGTIN
I3146-5ML04061838108593

Questions

1–5 of 5 Questions  
  1. 培地で希釈した1×Solutionは冷蔵でどのくらい保管できますか?

    1 answer
    1. The 100x stock solution of this product has a 2-year shelf life when stored at 2-8°C. However, stability of the solution after dilution or after opening has not been tested.

      Helpful?

  2. Can this ITS be diluted in DMEM medium containing phenol red

    1 answer
    1. This product is compatible with DMEM containing phenol red.

      Helpful?

  3. What should you use to dilute this to a 1X solution?

    1 answer
    1. This product can be dissolved in a given target cell culture medium, e.g., non-complex media (MEM, RPMI-1640), to a 1X solution.

      Helpful?

  4. What are the product numbers for the stand alone components in I3146?

    1 answer
    1. The product numbers for the stand-alone components in I3146 are as follows:
      Insulin – I2643 or I3536 (sterile)
      Apo-Transferrin – T2252
      Sodium Selenite – S5261
      EBBS – E3024

      Helpful?

  5. The ITS product #I3146 has been on back order for a while. Is there an alternative with similar components?

    1 answer
    1. Based on the Media Supplement Formulation, the closest alternative to product No. I3146 is the I2521 ITS+1 Liquid Media Supplement (100×), which also contains BSA and Linoleic acid.

      Helpful?

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