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H6909

Hydrocortisone solution

50 μM, sterile-filtered, BioXtra, suitable for cell culture

Sinónimos:

17-Hydroxycorticosterone, Cortisol

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Tamaño de envaseSKUDisponibilidadPrecio
10 mL
Comprobar disponibilidad del carrito
$72.200

Acerca de este artículo

Número CAS:
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352209
MDL number:
Biological source:
non-animal source
Form:
liquid

$72.200


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biological source

non-animal source

Quality Level

sterility

sterile-filtered

product line

BioXtra

form

liquid

concentration

50 μM

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

5.0- 7.0

shipped in

dry ice

storage temp.

−20°C

SMILES string

C[C@]12CCC(=O)C=C1CC[C@H]3[C@@H]4CC[C@](O)(C(=O)CO)[C@@]4(C)C[C@H](O)[C@H]23

InChI

1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1

InChI key

JYGXADMDTFJGBT-VWUMJDOOSA-N

Application

Hydrocortisone has been used in epithelial and endothelial adherent cell culture applications. Hydrocortisone solution has been used for the isolation of epidermal stem cells and cell expansion.[1] It has also been used for keratinocyte permeability assay.[2]

Physical form

frozen in working aliquots, avoid repeated freeze/thaw

Other Notes

Animal-component free

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Este artículo
H0888H3160H4001
biological source

non-animal source

biological source

soybean

biological source

plant (wild yam)

biological source

synthetic (organic)

Quality Level

500

Quality Level

200

Quality Level

300

Quality Level

300

product line

BioXtra

product line

BioReagent

product line

-

product line

-

concentration

50 μM

concentration

-

concentration

-

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

form

liquid

form

powder

form

powder

form

powder


Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Número de artículo de comercio global

SKUGTIN
H6909-10ML04061838259646

Questions

1–9 of 9 Questions  
  1. Regarding product H6909, I would like to know the name of the components in which it is diluted and its final concentration.

    1 answer
    1. Product No. H6909 is a solution of hydrocortisone in normal saline (0.85%).

      Helpful?

  2. Dear Madam/Sir, I hope you’re doing well. I am reaching out to ask about the composition of the cortisol solution at a concentration of 50uM. Could you confirm whether the cortisol is dissolved in water, ethanol, or another solvent? Thank you

    1 answer
    1. This product is 50 um Hydrocortisone solution prepared in saline. Ethanol is used to solubilize during preparation. The final ethanol concentration is approximately 0.2%.

      Helpful?

  3. How long is this product stable in cell culture medium (e.g. DMEM) at 4°C, or just 4°C in general?

    1 answer
    1. The stability of this product after being diluted in medium, has not been evaluated. However, various medium products that are supplemented with Hydrocortisone, are stable for up to 6 weeks when stored refrigerated. As an example, see product C-20011, Keratinocyte Growth Medium, at the link below:
      https://www.sigmaaldrich.com/US/en/product/sigma/c20011

      Helpful?

  4. Is it suitable to further dilute small quantities of Product H6909, Hydrocortisone solution, in phosphate-buffered saline to prepare lower concentration aliquots?

    1 answer
    1. It is not recommended to further dilute H6909, Hydrocortisone solution, for storage purposes as stability/integrity of more dilute forms cannot be guaranteed.

      Helpful?

  5. What is the concentration of Product No. H6909, Hydrocortisone solution?

    1 answer
    1. Product No. H6909 is a 50 μM solution. This may also be written as approximately 0.0181 g/L.

      Helpful?

  6. Should Product No. H6909, Hydrocortisone solution, be protected from light?

    1 answer
    1. Yes. Product No. H6909 is packaged in an amber vial.  If making single use aliquots, we recommend to use amber vials to protect the solution from light.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. Is there ethanol in Product No. H6909, Hydrocortisone solution?

    1 answer
    1. Hydrocortisone is initially solubilized in ethanol and then diluted in normal saline.  The final concentration of ethanol is 2 ml/L (0.2% v/v).

      Helpful?

  9. Is Product No. H6909, Hydrocortisone solution, isotonic?

    1 answer
    1. Product No. H6909 is a solution of hydrocortisone in normal saline (0.85%).  It is an isotonic solution of hydrocortisone.

      Helpful?

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