The GALS kit, identified by product number F1797, is not tested or recommended for use with a mouse brain that has been fixed using a different fixative for 4 days or longer. The standard kit procedure fixes cells for only 10 minutes, whereas the fixation period used for the mouse brain is 396 times longer than what is recommended in the kit protocol. The kit was never tested for use on a mouse brain fixed using another fixative. Therefore, it is uncertain whether the fixation used for the mouse brain might produce acceptable results
GALS
β-Galactosidase Reporter Gene Staining Kit
Sinónimos:
β-galactosidase, LacZ
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 1 kit | Comprobar disponibilidad del carrito | $371.000 |
Acerca de este artículo
NACRES:
NA.32
UNSPSC Code:
12161503
Storage temp.:
−20°C
Shipped in:
dry ice
$371.000
Comprobar disponibilidad del carrito
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleusage
kit sufficient for 100 tests (using a 3.5 cm dish)
Quality Segment
technique(s)
microbe id | staining: suitable
shipped in
dry ice
storage temp.
−20°C
General description
β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.
Application
β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as:
- sagittal brain sections in histoenzymology
- pancreas or colon sections in β-galactosidase reporter gene assay
- mice E10.5 -11.5 embryo and embryo sections
Other Notes
The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.
1 of 1
Este artículo | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - |
| shipped in dry ice | shipped in dry ice | shipped in ambient | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| usage kit sufficient for 100 tests (using a 3.5 cm dish) | usage kit sufficient for 100 histochemical assays | usage - | usage kit sufficient for 100 assays |
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
supp_hazards
Clase de almacenamiento
3 - Flammable liquids
flash_point_f
132.8 °F
flash_point_c
56 °C
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Contenido relacionado
General Information
Wendy Phillips et al.
Methods in molecular biology (Clifton, N.J.), 549, 137-155 (2009-04-21)
Animal models of neurodegenerative disease are excellent tools for studying pathogenesis and therapies including cellular transplantation. In this chapter, we describe different models of Huntington's disease and Parkinson's disease, stereotactic surgery (used in creation of lesion models and transplantation) and
beta-Galactosidase assay
Smale ST
Cold Spring Harbor Protocols, 2010, pdb-prot5423 (2010)
Snir Yehuda et al.
Biogerontology, 18(4), 525-533 (2017-03-03)
The role of telomere shortening in the induction of replicative cellular senescence (CS) is well known and as a result, the involvement of telomerase and in particular its catalytic subunit, the telomerase reverse transcriptase (TERT) in CS has also been
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 10651745001 | 04061838674142 |
| 10745740001 | 04061838675156 |
| B2904-500MG | 04061833427194 |
| 9660-1GM | 04055977229400 |
| 10703729001 | 04061838674487 |
| 11680293001 | 04061838704610 |
| 3117073001 | 04061826681725 |
| 9630-100MG | 04055977229394 |
| B2904-100MG | 04061833427187 |
| B8931-25MG | 04061833074022 |
| APREST85371-100UL | 04061835871742 |
| OGS391-5UG | 04061837170997 |
| OGS255-5UG | 04061837181511 |
| BG-3-G | 04053252270888 |
| GNZ-0024A-PA | 04065266093292 |
| GNZ-0023-BM | 04065266093186 |
| GNZ-0024A-BM | 04065266093261 |
| 71077-3 | 04055977271676 |
| SMB00401-25MG | 04061833052112 |
| GNZ-0024-BM | 04065266093223 |
| GNZ-0024-PA | 04065266093254 |
-
Is the GALS kit compatible with fixed, frozen mouse brain that used a totally different fixative for 4 days or longer?
1 answer-
Helpful?
-