FITC is not membrane permeable in healthy cells, it binds to surface proteins. Conjugates such as FITC-Dextran is an example of a more cell-permeable option.
F7250
Fluorescein isothiocyanate isomer I
≥90% purity (HPLC), powder
Sinónimos:
FITC, Fluorescein 5-isothiocyanate
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50 MG
$59.300
100 MG
$89.700
250 MG
$149.000
500 MG
$255.000
1 G
$446.000
5 G
$1.870.000
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50 MG
$59.300
100 MG
$89.700
250 MG
$149.000
500 MG
$255.000
1 G
$446.000
5 G
$1.870.000
About This Item
Fórmula empírica (notación de Hill):
C21H11NO5S
Número de CAS:
Peso molecular:
389.38
Beilstein:
1407295
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
NACRES:
NA.47
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Nombre del producto
Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
Nivel de calidad
Ensayo
≥90% (HPLC)
Formulario
powder
técnicas
titration: suitable
color
orange to dark orange
mp
>360 °C (lit.)
solubilidad
acetone: 1 mg/mL
fluorescencia
λex 492 nm; λem 518 nm (green)
idoneidad
suitable for protein labeling
aplicaciones
diagnostic assay manufacturing
hematology
histology
temp. de almacenamiento
2-8°C
cadena SMILES
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
Clave InChI
MHMNJMPURVTYEJ-UHFFFAOYSA-N
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Descripción general
Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[1][2]
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.[3]
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[1][2]
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.[3]
Aplicación
El reactivo para el marcado con FITC de proteínas; microsecuenciado de proteínas y péptidos (HPLC)
Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins[4][5][6], a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.
Biological applications include use as a fluorescent labeling reagent for proteins[4][5][6], a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1 - Skin Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Los clientes también vieron
Vaccines: Recent Trends and Progress (2012)
A Ramseier et al.
Electrophoresis, 19(16-17), 2967-2975 (1998-12-31)
Amines can easily be derivatized with fluorescein isothiocyanate isomer I (FITC) and analyzed by capillary electrophoresis (CE) using alkaline buffers with or without dodecyl sulfate micelles. This paper reports the CE analysis of FITC-derivatized amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and beta-phenylethylamine in
Structural mechanisms of acute VEGF effect on microvessel permeability.
Fu BM and Shen S
American Journal of Physiology. Heart and Circulatory Physiology, 284, H2124-H2124 (2003)
Chromatographically pure fluorescein and tetramethylrhodamine isothiocyanates.
L C FELTON et al.
Analytical biochemistry, 2, 178-180 (1961-04-01)
Human monoclonal autoantibodies specific for the bullous pemphigoid antigen 1 (BPAg 1).
Peyron E
Journal of Immunology, 153, 1333-1333 (1994)
Artículos
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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Are cell membranes of living cells permeable for FITC?
1 answer-
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Is the intensity of FITC (e.g., Product No. F7250) pH dependent?
1 answer-
Yes; many articles have reported the pH dependence of the fluorescence of fluorescein derivatives. See the following references: Archives Biochem. Biophys., 133, 263 (1969); J. Immunol., 95, 1165 (1966); Archives Biochem. Biophys., 73, 1 (1958); Standard test for labeling efficiency of FITC with BSA: NCCLS Approved Standard: ASM-1, 2nd ed., June, 1975; Characterization of FITC-dyes by IR and TLC: Analytical Biochem., 29, 526 (1969); Fluorescent labeling of proteins: Proc. Nat. Acad. Sci., 72, 459 (1975); Amer. J. Pathol., 34, 1081 (1958); Fluorescent labeling of a lectin: Meth. Enzymol., 81, 65 (1982).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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After the FITC (e.g., Product No. F7250) is conjugated to the protein, what is the solution stability of the conjugated protein?
1 answer-
The stability of the conjugated protein will be essentially the stability of the unconjugated protein. Some conjugated proteins might be stable when stored at -20°C and other conjugated proteins will have better solution stability at 2-8°C If the protein concentration is too low, this also has a negative effect upon the stability of the conjugated protein.
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Is Product No. F7250, Fluorescein isothiocyanate isomer I, pharmaceutical grade?
1 answer-
No. The product is considered suitable for research use only.
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How stable is FITC Isomer 1 (e.g., Product No. F7250) once conjugated to an antibody?
1 answer-
FITC can photo bleach fairly rapidly. The prepared solution should be stored in a dark container and protected from light. When viewing the antibody staining under a microscope, it is advisable to use subdued fluorescent light when possible. If the fluorescent light stays at one position for too long, photo bleaching is noticeable.
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