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Merck

F5392

Sigma-Aldrich

Fibroblast Growth Factor-Basic from bovine pituitary

~90% (SDS-PAGE and N-terminal analysis), lyophilized powder, suitable for cell culture

Sinónimos:

FGF2, bFGF

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Fibroblast Growth Factor-Basic from bovine pituitary, FGF-Basic, suitable for cell culture

biological source

bovine pituitary glands

Quality Level

assay

~90% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

0.03-3.0 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

16.4 kDa

packaging

pkg of 1 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<0.5 EU/vial

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

bovine ... FGF2(281161)

General description

The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells. The FGF2 gene is mapped to cattle chromosome 17.

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:
  • In animal and human cell culture
  • In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells
  • In the generation of directly reprogramming fibroblast
  • In human glioma cell culture

Biochem/physiol Actions

Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.

Physical form

Lyophilized from a 0.2 μm filtered solution in sodium phosphate and sodium chloride containing 0.1 mg bovine serum albumin

Preparation Note

Purified glands by a modification of the method of Gospodarowicz, et al.

Analysis Note

The proliferative activity is tested in culture using fetal bovine heart endothelial cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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Visite la Librería de documentos

Stimulatory effects of fibroblast growth factor 2 on proliferation and migration of uterine luminal epithelial cells during early pregnancy
Lim W, et al.
Biology of Reproduction, 96(1), 185-198 (2016)
Single nucleotide polymorphism of FGF2 gene in Iranian Holstein proven bulls
Salehi A, et al.
Molecular Biology Research Communications, 4(1), 57-57 (2015)
Kathryn A Jacobs et al.
Methods in molecular biology (Clifton, N.J.), 1749, 51-58 (2018-03-12)
Endothelial cells have the capacity to shift between states of quiescence and angiogenesis. The early stage of angiogenesis, sprouting, occurs with the synchronized activities of tip cells, which lead the migration of the sprout, and stalk cells, which elongate this
Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: comparison of neural differentiation of rat AdMSCs and BMSCs
Capkin M, et al.
Biomedical Materials (Bristol, England), 7(4), 045013-045013 (2012)
Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors.
Wu W, et al.
Experimental and Therapeutic Medicine, 13(6), 2685-2690 (2017)

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