Yes. stock solutions in ethanol may be sterile filtered. However, cellulose-based filter membranes are not recommended for use with ethanol. Please see the link below to review additional information regarding filter membrane compatibility with commonly used solvents:
https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/chemical-compatibility-of-filter-components
E2758
β-Estradiol
BioReagent, powder, suitable for cell culture
Sinónimos:
1,3,5-Estratriene-3,17β-diol, 17β-Estradiol, 3,17β-Dihydroxy-1,3,5(10)-estratriene, Dihydrofolliculin
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
250 MG
$45.700
1 G
$113.000
5 G
$454.000
Seleccione un Tamaño
Cambiar Vistas
250 MG
$45.700
1 G
$113.000
5 G
$454.000
About This Item
Fórmula empírica (notación de Hill):
C18H24O2
Número de CAS:
Peso molecular:
272.38
Beilstein:
1914275
Número CE:
Número MDL:
Código UNSPSC:
12352209
ID de la sustancia en PubChem:
NACRES:
NA.77
origen biológico:
synthetic (organic)
Formulario:
powder
Ensayo:
≥98% (HPLC)
Productos recomendados
origen biológico
synthetic (organic)
Nivel de calidad
Línea del producto
BioReagent
Ensayo
≥98% (HPLC)
Formulario
powder
técnicas
cell culture | mammalian: suitable
single cell analysis: suitable
mp
176-180 °C (lit.)
solubilidad
ethanol: 50 mg/mL, clear, colorless
Condiciones de envío
ambient
temp. de almacenamiento
room temp
cadena SMILES
O[C@H]1CC[C@@]2([H])[C@]3([H])CCC4=CC(O)=CC=C4[C@@]3([H])CC[C@@]21C
InChI
1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17+,18+/m1/s1
Clave InChI
VOXZDWNPVJITMN-ZBRFXRBCSA-N
Información sobre el gen
human ... ESR1(2099) , ESR2(2100) , ESRRA(2101) , ESRRB(2103) , GPER(2852) , SERPINA6(866)
mouse ... Esr1(13982) , Esr2(13983) , Esrra(26379)
rat ... Afp(24177) , Ar(24208) , Esr1(24890) , Esr2(25149) , Shbg(24775)
¿Está buscando productos similares? Visita Guía de comparación de productos
Descripción general
β-Estradiol or estradiol-17 β (E2) is secreted majorly by the large preovulatory follicles in the ovary. In addition, estradiol can be synthesized de novo from cholesterol have androgens synthesized from cholesterol. The aromatization of androgen by enzyme aromatase (P450arom) results in the β-estradiol production.[1]
Aplicación
β-Estradiol has been used:
- as a component of Dulbecco′s modified Eagle medium (DMEM) for culturing mammary tumor cells[2]
- as a oestrogenic compound for testing neuroprotective effects[3]
- as a component of medium199 for culturing oocytes[4]
β-Estradiol is used to study cell differentiation and transformations (tumorigenicity).
Acciones bioquímicas o fisiológicas
The major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female phenotype in embryogenesis and during puberty by regulating gene transcription and, thus, protein synthesis. It also induces the production of gonadotropins which, in turn, induce ovulation. Exposure to estradiol increases breast cancer incidence and proliferation.
β-Estradiol (E2) is a major premenopausal hormone in women and is essential for spermiogenesis, sperm maturation and motility in men.[5] Cultured bone marrow stem cells (BMSCs) have ability to synthesize E2 and could be exploited for treating estrogen deficiency.[1] It mediates physiological functions through estrogen receptor (ER) α and ERβ.[6]
The major estrogen secreted by the premenopausal ovary.
Forma física
powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw
Reconstitución
To prepare a 20 μg/ml stock solution, add 1 ml absolute ethanol to 1 mg β-estradiol; gently swirl to dissolve; add 49 ml sterile medium while mixing.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Lact. - Repr. 1A
Código de clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Loss of STAT1 from mouse mammary epithelium results in an increased Neu-induced tumor burden
Klover PJ, et al.
Neoplasia, 12(11), 899-905 (2010)
Selective oestrogen receptor (ER) modulators reduce microglia reactivity in vivo after peripheral inflammation: potential role of microglial ERs
Tapia-Gonzalez S, et al.
The Journal of Endocrinology, 198, 219?230-219?230 (2008)
Molecular mechanism of estrogen-estrogen receptor signaling
Yacsar P, et al.
Reproductive medicine and biology, 16(1), 4-20 (2017)
Estrogen synthesis and signaling pathways during aging: from periphery to brain
Cui J, et al.
Trends in Molecular Medicine, 19(3), 197-209 (2013)
Estradiol synthesis and release in cultured female rat bone marrow stem cells
Zhang D, et al.
BioMed Research International, 2013 (2013)
-
Can Estradiol/ethanol solutions be sterile filtered?
1 answer-
Helpful?
-
-
Hello, I would like to check the solubility of this product in DMSO. Best,
1 answer-
The solubility of this product in DMSO has not been determined. However, as per the literature, the β-Estradiol is soluble in DMSO at 20 mg/ml.
Helpful?
-
-
after dissolving the β-Estradiol in ethanol , whats the tempreture to store the item in ?
1 answer-
The storage or stability conditions once the compound is in solution is not determined. General recommendations for β-Estradiol are to protect from light, and store at -20C or at 2-8C in a degassed solution. Solutions stored in this manner should remain stable in the range of 1 month - 1 year.
Helpful?
-
-
How diluit for concentration of the 100ng/mL
1 answer-
β-Estradiol is soluble in absolute ethanol at 50 mg/mL (See E8875). Preparing a 100 ng/mL solution would require serial dilutions.
Start with a 1 mg/mL (1000 ug/mL) stock in ethanol.
Dilute 10 uL (10 ug = 10,000 ng) of this stock with 9.990 mL to achieve a 1000 ng/mL solution. This can be diluted with medium or buffer.
Dilute 1 mL of this with 9 mL to the final concentration of 100 ng/mL.Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
