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D3812

DirectLoad™ 50 bp DNA Step Ladder

ready-to-use marker for DNA electrophoresis

Sinónimos:

DirectLoad™ 50 bp DNA Marker, Ready-to-use 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Step Ladder

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Tamaño de envaseSKUDisponibilidadPrecio
1 vial
Comprobar disponibilidad del carrito
$164.000

Acerca de este artículo

NACRES:
NA.25
UNSPSC Code:
41105335

$164.000

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Quality Level

200

form

(liquid)

usage

sufficient for 100 loads

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s DirectLoad™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
DirectLoad™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s DirectLoad™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

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Este artículo
S7025D3687D3937
DirectLoad™ 50 bp DNA Step Ladder ready-to-use marker for DNA electrophoresis

Sigma-Aldrich

D3812

DirectLoad™ 50 bp DNA Step Ladder

50 bp DNA Step Ladder for DNA electrophoresis

Sigma-Aldrich

S7025

50 bp DNA Step Ladder

DirectLoad™ PCR 100 bp Low Ladder ready-to-use marker for DNA electrophoresis

Sigma-Aldrich

D3687

DirectLoad™ PCR 100 bp Low Ladder

DirectLoad™ 1 kb DNA Ladder ready-to-use marker for DNA electrophoresis

Sigma-Aldrich

D3937

DirectLoad™ 1 kb DNA Ladder

form

(liquid)

form

liquid

form

liquid

form

liquid

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

usage

sufficient for 100 loads

usage

100 uses

usage

75 uses

usage

100 uses

NA electrophoresis

NA electrophoresis, ladder, marker

-

NA electrophoresis, ladder, marker

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Clase de almacenamiento

12 - Non Combustible Liquids

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Certificados de análisis (COA)

Lot/Batch Number
SLCL7565
SLCK4692
SLCD0173
SLBW4614
SLBT0918

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Protocolos

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Artículos

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Contenido relacionado

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Número de artículo de comercio global

SKUGTIN
D3812-1VL04061835515967

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