C9282

Colato de sodio hydrate

suitable for cell culture, BioReagent

• Sinónimos: Ácido 3α,7α, 12α-trihidroxi-5β-colan-24-oico sodium salt, Ácido colálico sodium salt
• Fórmula empírica (notación de Hill): C₂₄H₃₉NaO₅ · xH₂O
• Número CAS: 206986-87-0
• Peso molecular: 430.55 (anhydrous basis)
• UNSPSC Code: 12161902
• NACRES: NA.75
• PubChem Substance ID: 24893147
• EC Number: 206-643-5
• Beilstein/REAXYS Number: 3582354
• MDL number: MFCD00150749

Propiedades

• Quality Segment: 200
• biological source: bovine bile, ovine bile
• description: anionic
• product line: BioReagent
• assay: ≥99.0% (HPLC)
• form: powder
• mol wt: micellar avg mol wt 900-1300
• aggregation number: 2-3
• technique(s): cell culture | mammalian: suitable
• CMC: 9-15 mM (20-25°C)
• solubility: water: 100 mg/mL, clear, colorless to light yellow
• HLB: 18
• SMILES string: O.[Na+].C[C@H](CCC([O-])=O)C1CCC2C3[C@H](O)CC4C[C@H](O)CC[C@]4(C)C3C[C@H](O)[C@]12C
• InChI: 1S/C24H40O5.Na.H2O/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26;;/h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29);;1H2/q;+1;/p-1/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-;;/m1../s1
• InChI key: MUVVIYFKOVLQHL-RCVKHMDESA-M

Descripción

General description

Cholate is a primary bile acid produced from cholesterol in the liver.[1] It is an anionic detergent synthesized from cholesterol in the presence of sterol 12α-hydroxylase (CYP8B1).[2]

Application

Cholate is a bile acid produced in liver during cholesterol breakdown. Cholate downregulates cholesterol-7-α-hydroxylase (rate-limiting step in bile acid synthesis). Cholate, an anionic detergent, is used alone and in combination with urea to extract and reconstitute membrane proteins and protein complexes.

Sodium cholate hydrate has been used:

• as a bile acid to induce bile acid-responsive (BEAR) transcription system[3],
• as a component of Campylobacter defined broth (CDB) to test its effect on stress response in Campylobacter jejuni{52
• to test its sensitivity towards the bile acid sensor[4]

Biochem/physiol Actions

Reduction in 12-α hydroxylase activity leads to low levels of cholic acid in liver disease.[1]

Información de seguridad

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)