A8181

Amido Black Staining Solution 2X

electrophoresis reagent

• Sinónimos: Naphthol Blue Black solution, Amido Black 10B
• Fórmula empírica (notación de Hill): C₂₂H₁₄N₆Na₂O₉S₂
• Número de CAS: 1064-48-8
• Peso molecular: 616.49
• Beilstein/REAXYS Number: 5374263
• MDL number: MFCD00004017
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891210
• NACRES: NA.32

Propiedades

• form: liquid
• SMILES string: [Na+].[Na+].Nc1c(\N=N/c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\C=C/c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
• InChI key: AOMZHDJXSYHPKS-DROYEMJCSA-L

Descripción

General description

Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.

Application

Amido Black Staining Solution 2X has been used in the staining:

• serum proteins present in the culture supernatant
• cysteine sulfinic acid decarboxylase protein in mouse liver homogenate
• histones from chromatin samples

Reconstitution

After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.

Información de seguridad

• pictograms: GHS02,GHS07
• signalword: Danger
• hcodes: H225,H315,H319,H336
• pcodes: P210 - P233 - P240 - P241 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Central nervous system
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 1
• flash_point_f: 53.6 °F
• flash_point_c: 12 °C