A0157
Ascorbate Oxidase from Cucurbita sp.
lyophilized powder, 1,000-3,000 units/mg protein
Sinónimos:
L-Ascorbate:oxygen oxidoreductase, Ascorbase
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 100 units | Comprobar disponibilidad del carrito | $48.200 | |
| 250 units | Comprobar disponibilidad del carrito | $127.000 | |
| 1000 units | Comprobar disponibilidad del carrito | $267.000 |
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-852-6
MDL number:
Specific activity:
1,000-3,000 units/mg protein
Biological source:
plant (Cucurbita spp.)
$48.200
Comprobar disponibilidad del carrito
Servicio técnico
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Permítanos ayudarlebiological source
plant (Cucurbita spp.)
Quality Segment
form
lyophilized powder
specific activity
1,000-3,000 units/mg protein
storage temp.
2-8°C
General description
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.
Application
Ascorbate Oxidase from Cucurbita sp. has been used:
- as a supplement in the culture medium for differentiation into osteoblasts
- to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay
- in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations
Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis [1].
Biochem/physiol Actions
Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.
Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.
Physical form
Lyophilized powder containing buffers and sucrose as stabilizer.
Other Notes
One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.
1 of 1
Este artículo | |||
|---|---|---|---|
| biological source plant (Cucurbita spp.) | biological source - | biological source bacterial (Aerococcus spp.) | biological source plant (Cucurbita species) |
| specific activity 1,000-3,000 units/mg protein | specific activity ≥1500 U/mg, ≥300 units/mg material | specific activity ≥35 units/mg protein (biuret) | specific activity 17 units |
| form lyophilized powder | form lyophilized | form lyophilized powder | form solid |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| A0157-100UN | 04061833326985 |
| A0157-1KU | 04061833326992 |
| A0157-250UN | 04061833327005 |