This product is a calcium chloride solution for molecular biology; however, it is not sterile. Product C-34006 is a sterile calcium chloride solution.
21115
Cloruro de calcio solution
BioUltra, Molecular Biology, ~1 M in H2O
Calcium chloride solution
Sinónimos:
CaCl2, Calcium chloride hydrate, Calcium dichloride
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 1 mL | Comprobar disponibilidad del carrito | $84.200 | |
| 100 mL | Comprobar disponibilidad del carrito | $155.000 | |
| 250 mL | Comprobar disponibilidad del carrito | $320.000 |
Acerca de este artículo
Fórmula lineal:
CaCl2
Número CAS:
Peso molecular:
110.98
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12352302
MDL number:
Grade:
Molecular Biology
Form:
liquid
Servicio técnico
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Molecular Biology
Quality Segment
product line
BioUltra
form
liquid
concentration
~1 M in H2O
impurities
DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
refractive index
n20/D 1.358
pH
4.0-6.0 (25 °C)
density
1.086 g/mL at 20 °C
cation traces
Ba: ≤1 mg/kg, Bi: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤1 mg/kg, Mg: ≤5 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤20 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤20 mg/kg, Zn: ≤1 mg/kg
λ
neat
UV absorption
λ: 260 nm Amax: 0.04, λ: 280 nm Amax: 0.02
SMILES string
Cl[Ca]Cl
InChI
1S/Ca.2ClH/h;2*1H/q+2;;/p-2
InChI key
UXVMQQNJUSDDNG-UHFFFAOYSA-L
Application
Calcium chloride solution has been used in hippocampal slice culture preparation and HEPES-buffered tyrode′s solution.
Other Notes
In electric-field induced DNA transfection - increased by millimolar concentrations of calcium chloride[1].
1 of 1
Este artículo | |||
|---|---|---|---|
| form liquid | form solid | form granular | form granular |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
| grade Molecular Biology | grade - | grade anhydrous | grade anhydrous |
| pH 4.0-6.0 (25 °C) | pH 8-10 (20 °C, 100 g/L in H2O) | pH - | pH - |
| density 1.086 g/mL at 20 °C | density 2.15 g/cm3 at 20 °C | density - | density - |
| impurities DNases, none detected, insoluble matter, passes filter test, proteases, none detected, RNases, none detected, phosphatases, none detected | impurities ≤0.2% Free alkali (as Ca(OH)2) | impurities - | impurities - |
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves
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Protocolos
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
Insulin-like growth factor-1 abrogates microglial oxidative stress and TNF-alpha responses to spreading depression
Grinberg Y Y, et al.
Journal of Neurochemistry, 126(5), 662-672 (2013)
T D Xie et al.
Biophysical journal, 58(1), 13-19 (1990-07-01)
A study of mechanisms of electrotransfection using Escherichia coli (JM 105) and the plasmid DNA pBR322 as model system is reported. pBR322 DNA carries an ampicillin resistance gene: E. coli transformants are conveniently assayed by counting colonies in a selection
Fluid osmolarity acutely and differentially modulates lymphatic vessels intrinsic contractions and lymph flow
Solari E, et al.
Frontiers in Physiology, 871-871 (2018)
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 21115-1ML | 04061838090003 |
| 21115-100ML | 04061838771582 |
| 21115-250ML | 04061838771599 |
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is it sterile?
1 answer-
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Is this material classified as endotoxin free?
1 answer-
This product is not tested for Endotoxin. Currently, there is only 1 Calcium Chloride product that is tested for Endotoxin, product 1371015000. This is a powder form with a maximum Endotoxin specification of 2.5 I.U./gram. Please see the link below to review this product option:
https://www.sigmaaldrich.com/product/sigma/21115Helpful?
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What is the purity percentage?
1 answer-
Purity testing is not performed on this solution. Testing includes trace analysis and verification of the concentration. However, the Calcium Chloride used to prepare this solution has a minimum purity of 99.5%.
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How long is this stable at room temperature in an unopened state?
1 answer-
This product may be stored in room temperature. Concerning expiration, this product is assigned a product retest date. Products that, while considered stable, may be subject to degradation over time are assigned a periodic retest date to confirm quality. The lot-specific retest date is reported on the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/21115#product-documentationHelpful?
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what is the storage temperature for this?
1 answer-
This product is stored tightly closed at room temperature.
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Is this suitable for cell culture?
1 answer-
The item is tested for suitability in molecular biology applications. It has not been tested for use in cell culture. Please see the link below to review product C-34006, Calcium Chloride Solution, which is suitable for cell culture use:
https://www.sigmaaldrich.com/product/sigma/c34006Helpful?
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