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Merck

11557

Sigma-Aldrich

Ácido 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfónico) diammonium salt

chromogenic, ≥98% (HPLC), powder or crystals

Sinónimos:

AzBTS-(NH4)2, Diamonio del 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfonato)

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About This Item

Fórmula empírica (notación de Hill):
C18H24N6O6S4
Número de CAS:
Peso molecular:
548.68
Beilstein/REAXYS Number:
7329461
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83

product name

Ácido 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfónico) diammonium salt, ≥98% (HPLC)

Quality Level

assay

≥98% (HPLC)

form

powder or crystals

impurities

≤6.0% water

solubility

H2O: 10 mg/mL, slightly hazy to clear

storage temp.

2-8°C

SMILES string

[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

InChI

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

InChI key

OHDRQQURAXLVGJ-AXMZSLBLSA-N

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Application

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used:
  • in the radical scavenging assay to test the antioxidant property of Pinus densiflora bark (PDB) extracts
  • to test the antioxidant capacity (AOC) of Aloe vera samples
  • as a substrate for streptavidin-horse radish peroxidase (HRPO) in enzyme-linked immunosorbent assay (ELISA)

El ácido 2,2′-acino-bis(3-etilbenzotiazolina-6-sulfónico es un sustrato de la peroxidasa adecuado para procedimientos ELISA. Este sustrato rinde un producto final soluble que es de color verde y puede leerse espectrofotométricamente a 405 nm. La reacción puede pararse con dodecilsulfatosódico (SDS) al 1 %. Recomendado para procedimientos ELISA (micropocillo), no recomendado para aplicaciones con membrana.

Other Notes

Chromogenic substrate for peroxidase; The ABTS-H2O2-peroxidase system in EIA

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


Certificados de análisis (COA)

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Visite la Librería de documentos

A Szutowicz et al.
Analytical biochemistry, 138(1), 86-94 (1984-04-01)
Monoamine oxidase is assayed in tissue by a colorimetric reaction using horse radish peroxidase and 2,2'-azinodi(ethylbenzthiazoline-6-sulfonic acid to measure H2O2 formed during oxidation of amines. The method has a coefficient of variation of approximately 2.5% and provides results comparable with
Antioxidant and cytotoxicological effects of Aloe vera food supplements
Lopez Z, et al.
Journal of Food Quality, 2017 (2017)
Prathik Roy et al.
Journal of hazardous materials, 243, 286-291 (2012-11-21)
We have developed a simple, colorimetric iron telluride (FeTe) nanorods (NRs) based system for the detection of mercury, mainly based on the cation exchange reaction between FeTe NRs and Hg(2+). FeTe NRs (length, 105 ± 21 nm) react with Hg(2+)
A Catarina Guedes et al.
Food chemistry, 138(1), 638-643 (2012-12-26)
A renewed interest in antioxidants has arisen in recent years; microalgae and cyanobacteria are potential sources thereof for use as food/feed ingredients. However, improved methods for comprehensive screening of antioxidant capacity specifically in intracellular extracts of marine microorganisms are required
Thamizhiniyan Venkatesan et al.
BioMed research international, 2019, 3520675-3520675 (2019-08-31)
It is well established that various extraction factors, including the method, temperature, time, and solvent system, significantly influence the antioxidant quality of plant-derived products. Previously, we observed that extraction of Pinus densiflora bark (PDB) by the most common traditional Soxhlet

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