A-004-M

Poly-ʟ-Ornithine

synthetic, liquid, 0.1 mg/mL, suitable for cell culture

• Sinónimos: Poly-L-Ornithine Solution (0.01%), A-004-M, Sigma-Aldrich
• UNSPSC Code: 12352209
• eCl@ss: 32160801
• NACRES: NA.75
• Biological source: synthetic
• Form: liquid
• Technique(s): cell attachment: suitable, cell culture | mammalian: suitable
• Concentration: 0.01 % (w/v), 0.1 mg/mL
• Mol wt: M_w 78000 Da

Propiedades

• Nombre del producto: Poly-L-Ornithine Solution (0.01%),
• biological source: synthetic
• Quality Level: 100
• needle size: 2.0 mm
• form: liquid
• mol wt: M_w 78000 Da
• manufacturer/tradename: NovaSeptum^®
• concentration: 0.01 % (w/v), 0.1 mg/mL
• technique(s): cell attachment: suitable, cell culture | mammalian: suitable

Descripción

General description

Research Category: Stem Cell Research

Research Sub Category: Adhesion (CAMs)

Application

Poly-L-Ornithine Solution (0.01%) has been used to coat:

• glass-bottom plates for cell plating in a research study investigating the localization of cMET in MDA-MB-468 cells using confocal microscopy
• culture dishes for adult human neural stem cells (ahNSCs) primary culture[1]
• 6-well plates for induced pluripotent stem cells (iPSCs) culture and neuronal differentiation[2]

Physical form

Product is presented in sterile water at 0.01% w/v (0.1mg/ml). Product is filtered through a 0.2 micron filter before freezing.

Preparation Note

1. Thaw Poly-L-Ornithine solution at room temperature.

2. Dilute Poly-L-Ornithine solution to the desired concentration in sterile water.

3. Fully coat the cell culture surface with diluted Poly-L-Ornithine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.

4. Allow the cell culture vessel to sit at room temperature overnight.

5. Aspirate the Poly-L-Ornithine solution the following day and rinse the vessel with sterile water followed by coating with desired ECM protein. Optimal coating concentrations must be determined by the end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.

Store at -20°C . Avoid multiple freeze thaw cycles to maintain product integrity.

Analysis Note

Solution is clear with no particulates present. Solution is suitable for cell culture applications.

Legal Information

NOVASEPTUM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• wgk: WGK 1