70976
SpinPrep PCR Clean-up Kit
Rapid purification of PCR products for downstream procedures
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About This Item
UNSPSC Code:
41105500
NACRES:
NA.52
Productos recomendados
manufacturer/tradename
Novagen®
storage condition
OK to freeze
Storage temp.
10-30°C
General description
Rapid purification of PCR products for downstream procedures
The SpinPrep<TMSYMBOL></TMSYMBOL> PCR Clean-Up Kit is designedfor the rapid purification of DNA amplifiedin PCR. The 10-minute procedure involvesaddition of a binding buffer followed byadsorption of the DNA to a silica membrane ina spin column format. Following a wash step,the DNA is eluted in low-salt buffer. This kitremoves DNA polymerases, dNTPs, salts, and> 99% of primers so that they do not interferewith downstream applications such as cloning,sequencing, or labeling. PCR products from100 bp to > 12,000 bp can be cleaned up, withrecoveries of amplified DNA in the range of60-90% under standard conditions.
Column binding capacity: up to 6 g
PCR volume: 100 l/rxn
Typical recovery: 6090%
Size range: 100 bp to > 12,000 bp
Time required:< 10 min
Column binding capacity: up to 6 g
PCR volume: 100 l/rxn
Typical recovery: 6090%
Size range: 100 bp to > 12,000 bp
Time required:< 10 min
Components
•82 mlSpinPrep Bind Buffer
•27 mlSpinPrep Wash Buffer
•10 mlSpinPrep Elute Buffer
•100SpinPrep Filters
•100Receiver Tubes
•100Eluate Receiver Tubes
•27 mlSpinPrep Wash Buffer
•10 mlSpinPrep Elute Buffer
•100SpinPrep Filters
•100Receiver Tubes
•100Eluate Receiver Tubes
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
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Yong H Park et al.
Investigative ophthalmology & visual science, 57(2), 508-526 (2016-02-13)
The α-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) receptors (AMPAR) subunits can be posttranscriptionally modified by alternative splicing forming flip and flop isoforms. We determined if an ischemia-like insult to retinal ganglion cells (RGCs) increases AMPAR susceptibility to s-AMPA-mediated excitotoxicity through changes in posttranscriptional
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