This enzyme is a recombinant protein produced in e. coli. The biological source and sequence of the protein is considered proprietary and may not be shared.
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04529
Nitrilase
recombinant, expressed in E. coli, ≥2.0 U/mg
Synonym(e):
Nitrile aminohydrolase
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Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥2.0 U/mg
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
form
crystals, powder
specific activity
≥2.0 U/mg
mol wt
41 kDa
storage temp.
2-8°C
General description
Application
Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies.[3]
Biochem/physiol Actions
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥2.0 U/mg | specific activity ≥50 units/mg protein | specific activity ≥20,000 U/g | specific activity ≥130 units/mg protein |
| form crystals, powder | form lyophilized powder | form lyophilized | form buffered aqueous glycerol solution |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 41 kDa | mol wt 110 kDa | mol wt - | mol wt 32.5-33 kDa |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Shivani Jamwal et al.
International journal of biological macromolecules, 131, 117-126 (2019-03-08)
Immobilization of enzymes to improve their catalytic properties is an attractive protocol which makes them suitable candidates to meet various industrial demands. Present study describes the synthesis of new acryloyl crosslinked cellulose dialdehyde (ACCD) for nitrilase immobilization. Nitrilase was immobilized
Ahmed Kamal et al.
Journal of microbiology and biotechnology, 21(1), 37-42 (2011-02-09)
A new versatile acrylonitrile-bioconverting strain isolated from a petroleum-contaminated sludge sample and identified as Rhodococcus ruber AKSH-84 was used for optimization of medium and biotransformation conditions for nitrilase activity to produce acrylic acid. A simple and rapid HPLC protocol was
Alena Petříčková et al.
Applied microbiology and biotechnology, 93(4), 1553-1561 (2011-09-06)
Nitrilases from Aspergillus niger CBS 513.88, A. niger K10, Gibberella moniliformis, Neurospora crassa OR74A, and Penicillium marneffei ATCC 18224 were expressed in Escherichia coli BL21-Gold (DE3) after IPTG induction. N. crassa nitrilase exhibited the highest yield of 69,000 U L(-1)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 238430-50G | 04061838786890 |
| 238430-10G | 04061838347695 |
| 04529-50MG | 04061833227930 |
| 04529-10MG | 04061833227923 |
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what is the bacteria or organism whose genome sequence have been expressed in E.Coli for nitrilase procurement? I need the name of the organism for research purpose
1 Antwort-
Hilfreich?
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Can we get the source of the bacteria or organism whose genome sequence is being used for recombinant expressing in E.Coli for procuring nitrilase. Used for research purspose by a student
1 Antwort-
Unfortunately, the source and sequence is considered proprietary.
Hilfreich?
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Is the nitrilase been synthesized through DNA recombinant technology or is there any other source organism from which the nitrilase has been procured?
1 Antwort-
Yes, this product is a recombinant protein expressed in E. coli.
Hilfreich?
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