G8880

Glutaminase aus E. coli

Grade V, lyophilized powder, 50-200 units/mg protein

• Synonym(e): L-Glutamine amidohydrolase
• CAS-Nummer: 9001-47-2
• EC-Nummer: 3.5.1.2 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131193
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Escherichia coli
• Qualitätsniveau: 200
• Typ: Grade V
• Form: lyophilized powder
• Spezifische Aktivität: 50-200 units/mg protein
• Mol-Gew.: 110 kDa
• Zusammensetzung: Protein, ~30% biuret
• Kationenspuren: NH₄⁺: <0.1 μg/unit
• Fremdaktivität: NADH Oxidase ≤0.01%
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Glutaminase is an amidohydrolase.[1] It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain.[2]

Anwendung

Glutaminase from E.coli has been used:

• for the determination of glutamine from the cerebral cortex tissue extract[3]
• for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine[1]
• in combination with acivicin in in vitro melanoma cells[4] and MCF-7 and OAW-42 cells[5] for cell proliferation and invasiveness assay.

Biochem./physiol. Wirkung

Glutaminase catalyzes the conversion of glutamine to glutamate.

Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells.[4][5] Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden.[5]

Einheitendefinition

One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.

Physikalische Form

Lyophilized powder containing potassium succinate and EDTA

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)